Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections

Mahmoud Masoud, Salwa; Mahmoud Abd El-Baky, Rehab; A. Aly, Sherine; Ali Ibrahem, Reham

doi:10.21608/nrmj.2022.231801

Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections

Document Type : Original Article

Authors

¹ Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt

² Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 11566, Egypt

³ Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71516, Egypt

10.21608/nrmj.2022.231801

Abstract

Urinary tract infections are common human bacterial infections caused by Escherichia coli, which is one of the most frequent uropathogens. Treatment of such infections has become challenging due to the elevated levels of antimicrobial resistance. The aims of this work were to study the resistance pattern of E. coli pathogens recovered from patients with urinary tract infections attending to Minia University Hospital, Minia Governorate, Egypt, and to detect the co-existence of different extended spectrum β-lactamases (ESBLs) and metallo β-lactamases (MBLs) genes. The in vitro antibiotic sensitivity was tested using the disc diffusion assay. The production of ESBLs and MBLs was investigated through the combined disk and EDTA-combined disk synergy assays, while the molecular studies were carried out using the conventional polymerase chain reaction (PCR). High prevalence of ESBLs producers (61.6 %) was detected, whereas ESBLs and MBLs production was significantly associated with high multiple antimicrobial resistance index (MARI). The bla-_TEM was the most prevalent genotype (94.6 %), while the prevalence of bla-_NDM and bla-_IMP was 45.9 % and 40.5 %, respectively. Imipenem and amikacin were the most effective antibacterial antibiotics. The current results showed statistically significant association of the tested genes, as 89 % of the E. coli isolates harbored more than one gene. This study highlighted a public health concern; as the elevated antibiotic resistance narrowed the therapeutic options and led to the failure of treatment of such bacterial infections.

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