Document Type : Original Article
Authors
1
Department of Biological Sciences, Faculty of Basic and Applied Sciences, Elizade University, Ilara-Mokin, Ondo State, Nigeria
2
Department of Biological Sciences, University of Medical Sciences, Ondo State, Nigeria of medical sciences, ondo
3
Department of Microbiology, School of Life Sciences, Federal University of Technology, Akure, Ondo State, Nigeriaepartment of Microbiology, Federal University of Technology, Akure.
4
Department of Medical Laboratory Science, Achievers University, Owo, Ondo State. Nigeria
Abstract
Background: There is high incidence of nosocomial in developing countries and the bacteria responsible are becoming more resistant to commercially available antibiotics, hence, the need for this research. Methods: Isolation and identification of microorganisms from different wards of Ilara-Mokin health centers was done using standard methods. The bacteria isolated were identified and subjected to susceptibility test using four extracts and standard antibiotics for comparative study. Results: A high bacterial load of 52.00 ± 3.46 cfu/ml obtained was from the toilet in maternity ward, while the least bacterial load of 2.67 ± 0.67 cfu/ml was obtained from the floor of the male ward. A total of twelve (12) bacteria were isolated and identified, while nine (9) fungi were equally isolated and identified. The susceptibility of the bacteria isolates to ethanol extracts of castor, garlic, beniseed and bitter cola showed that garlic extract is most effective on the isolates having the highest diameter of zone of inhibition, Bacillus licheniformis with a diameter of 18.20±2.05 mm. Conclusion: The garlic ethanol extract was the most effective on the isolates while Pseudomonas was the most unsusceptible to all the extracts. The extracts tested in this work comparatively exceeded the potency of antibiotics, however, the Gram-negative bacteria are not very susceptible to the extracts. Therefore, these extracts can be used in development of novel antibiotics, especially to help curb resistance of pathogenic microorganisms implicated in nosocomial infections.
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