Evaluation of PRDM1 gene polymorphism with hepatitis C virus-related hepatocellular carcinoma in a cohort of Egyptian patients

Ibrahim, Aya M; Mohamed, Amal A; Esmail, Omnia E; Khater, Amir; El-Awady, Rehab R.

doi:10.21608/aijpms.2022.151773.1154

Evaluation of PRDM1 gene polymorphism with hepatitis C virus-related hepatocellular carcinoma in a cohort of Egyptian patients

Document Type : Original research articles

Authors

¹ Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo, Egypt

² Department of Biochemistry National Hepatology and Tropical Medicinal Research Institute, Cairo, Egypt

³ Department National Hepatology, Internal Medicine and Tropical Medicine Research Institute, Cairo, Egypt

⁴ Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Girls), Al-Azhar University, Egypt,

10.21608/aijpms.2022.151773.1154

Abstract

Abstract
The third most common cancer-related cause of death worldwide is hepatocellular carcinoma (HCC). and it is the sixth most common primary malignancy overall. Egypt had the hepatitis C virus’s highest prevalence (HCV). A critical topic of study is the association between HCV and HCC. PR domain zinc finger protein 1(PRDM1) functions as a tumor suppressor in B and T cells and is critical for plasma cell development and the exhaustion of T lymphocytes caused by cancer and chronic viral infections.
Aim: This study aimed to compare Egyptian patients with HCC to a control group to determine the allele frequencies and genotype of the rs1010273 PRDM1 gene polymorphism.
Method: 200 Egyptian patients (100 HCC and 100 control). All participants underwent laboratory evaluations, comprising hepatitis markers (HBsAg, anti-HCV-Ab), liver function tests, serum alpha-fetoprotein, and complete blood count. Using the TaqMan allelic discrimination assay method, the PRDM1 rs1010273 was genotyped.
Results: Unlike healthy controls, the HCC patients had a greater frequency of the GA genotype (43% vs. 20%) and the A allele (26.5% vs. 10%). A significant difference was found between the GA and A allele of PRDM1 genotype of HCC patients and controls (P<.001).
Conclusion: A statistically significant and greater frequency of the A allele and GA genotype was found in HCC patients compared to controls, indicating a relationship between the PRDM1 AA+GA gene polymorphism and the HCC Egyptian population. The A allele was assumed to be a risk factor for HCC (OR=11.212). SNP (rs1010273G/A) may be an optional valuable marker.

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