Present study evaluated the sensitivity of skin test and enzyme linked immunosorbent assay (ELISA) using antigen of Gnathostoma doloresi adult obtained from wild boars for immunodiagnosis of the human gnathostomiasis in Ecuador. Examinations were performed on 17 subjects clinically diagnosed as gnathostomiasis, 18 Ecuadorian controls and 10 Japanese controls. Based on the criteria of the positivity, 9 mm or more in diameter of the wheal size of the skin test, the positives were 14 (82.4%) out of 17 patients by the injection with 1 pg antigen. Using 10μg antigen, the positive rate in patients attained to 100%, but 3 (16.7%) out of 18 controls also showed positive reaction. There was a statistically significant correlation (r= 0.68, P< 0.01) between the wheal size in the skin test and the period from the appearance of the initial symptom. These results suggest that the skin test was highly sensitive in gnathostomiasis. When ELISA value of 0.4 or more was considered positive, 15 (93.8%) out of 16 were positive in patients, 5 (27.7%) out of 18 were positive in Ecuadorian controls and none in 10 Japanese controls. The study revealed that ELISA showed high sensitivity.