28Feb 2017

ENZYME ACTIVITY PROFILING OF EXO -β-1,4- GLUCANASE, ENDO-β-1,3- GLUCANASE AND PROTEASE IN STREPTOMYCES SPECIES FROM HIGHLAND, MIDLAND AND LOWLAND AREAS OF KERALA, INDIA.

  • Division of Microbiology, Jawaharlal Nehru Tropical Botanical Garden and Research Institute, Palode, Thiruvananthapuram, 695562 Kerala, India.
  • Department of Biotechnology and Biochemical Engineering, SCT College of Engineering, Thiruvananthapuram, 695518 Kerala, India.
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Variability in land use have substantial influence on biological properties of soil that persistently associates with soil quality, evaluated by microbial and biochemical indicators in soil. Kerala, located in the southwestern tip of India, geographically divided into eastern highland, central midland and western lowland. The natural classification of Kerala?s landmass is an excellent illustration for describing land use types and environmental changes. This study scrutinizes the comparative activities of exo-β-1,4-glucanase, endo-β-1,3-glucanase and protease in Streptomyces spp. from different highland, midland and lowland areas of Kerala. Soils collected from 7 different locations of each land area were analyzed for estimating enzyme activity by semi-quantitative and quantitative approaches. A significant fluctuation in exo-β-1,4-glucanase activity was observed in three land use systems, showed high activity in highland areas where natural vegetation occurs and significantly decreased to lowland regions, mostly arable areas. Number of Streptomyces isolates also revealed a decreasing trend from highland to lowland areas. pH, electrical conductivity (EC), soil organic carbon (SOC) and soil organic matter (SOM) were showed a significant correlation with the number of Streptomyces isolates. The variation of exo-β-1,4-glucanase activity from highland to lowland suggested the sensitivity of the enzyme as an ecological and a soil quality indicator, hence it can be used as a conspicuous indicator of environmental changes.

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[Lekshmi K. Edison, Reshma R. Anilkumar, Gayathri V. and Pradeep N. S. (2017); ENZYME ACTIVITY PROFILING OF EXO -β-1,4- GLUCANASE, ENDO-β-1,3- GLUCANASE AND PROTEASE IN STREPTOMYCES SPECIES FROM HIGHLAND, MIDLAND AND LOWLAND AREAS OF KERALA, INDIA. Int. J. of Adv. Res. 5 (Feb). 2431-2444] (ISSN 2320-5407). www.journalijar.com

Dr. N. S. Pradeep
Senior scientist, Division of microbiology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Trivandrum, Kerala

DOI:

Article DOI: 10.21474/IJAR01/3427      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3427

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