Skip to main content
SpringerLink
Log in

Determination of Menadione by Liquid Chromatography-Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring

  • Notes
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

This study aimed to develop a menadione (MD) determination method employing liquid chromatography–tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4–8.2%, with the corresponding recoveries equaling 90.5–109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. T. Okano, Y. Shimomura, M. Yamane, Y. Suhara, M. Kamao, M. Sugiura, and K. Nakagawa, J. Biol. Chem., 2008, 283, 11270.

    Article  CAS  PubMed  Google Scholar 

  2. Y. Hirota, N. Tsugawa, K. Nakagawa, Y. Suhara, K. Tanaka, Y. Uchino, A. Takeuchi, N. Sawada, M. Kamao, A. Wada, T. Okitsu, and T. Okano, J. Biol. Chem., 2013, 288, 33071.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. K. Nakagawa, Y. Hirota, N. Sawada, N. Yuge, M. Watanabe, Y. Uchino, N. Okuda, Y. Shimomura, Y. Suhara, and T. Okano, Nature, 2010, 468, 117.

    Article  CAS  PubMed  Google Scholar 

  4. H. H. Thijssen, L. M. Vervoort, L. J. Schurgers, and M. J. Shearer, Br J. Nutr., 2006, 95, 260.

    Article  CAS  PubMed  Google Scholar 

  5. A. Al Rajabi, J. Peterson, S. W. Choi, J. Suttie, S. Barakat, and S. L. Booth, J. Chromatogr B, 2010, 878, 2457.

    Article  Google Scholar 

  6. M. S. Elgawish, C. Shimomai, N. Kishikawa, K. Ohyama, M. Wada, and N. Kuroda, Chem. Res. Toxicol., 2013, 26, 1409.

    Article  CAS  PubMed  Google Scholar 

  7. F. Demirkaya-Miloglu, Y. Kadioglu, and O. Senol, Iran J. Pharm. Res., 2014, 13, 353.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Y. Suhara, M. Kamao, N. Tsugawa, and T. Okano, Anal. Chem., 2005, 77, 757.

    Article  CAS  PubMed  Google Scholar 

  9. R. Liu, M. Wang, and L. Ding, Talanta, 2014, 128, 51.

    Article  CAS  PubMed  Google Scholar 

  10. Y. Suhara, A. Murakami, K. Nakagawa, Y. Mizuguchi, and T. Okano, Bioorg. Med. Chem., 2006, 14, 6601.

    Article  CAS  PubMed  Google Scholar 

  11. R. Losito, C. A. Owen, and E. V. Flock, Biochemistry, 1967, 6, 62.

    Article  CAS  PubMed  Google Scholar 

  12. D. Di Monte, G. Bellomo, H. Thor, P. Nicotera, and S. Orrenius, Arch. Biochem. Biophys., 1984, 235, 343.

    Article  PubMed  Google Scholar 

  13. D. Shang, M. Kim, and M. Haberl, J. Chromatogr. A, 2014, 1334, 118.

    Article  CAS  PubMed  Google Scholar 

  14. D. D. Jain, G. Subbaiah, M. Sanyal, and P. A. Shrivastav, Talanta, 2007, 72, 80.

    Article  CAS  PubMed  Google Scholar 

  15. K. Matsuura, T. Ohmori, M. Nakamura, Y. Itoh, and K. Hirano, Biomed. Chromatogr., 2008, 22, 387.

    Article  CAS  PubMed  Google Scholar 

  16. L. Vlase, D. S. Popa, D. Muntean, and S. Leucuta, Sci. Pharm., 2008, 76, 663.

    Article  CAS  Google Scholar 

  17. K. B. Kim, K. A. Seo, S.-E. Kim, S. K. Bae, D. H. Kim, and J. G. Shin, J. Pharm. Biomed. Anal., 2011, 56, 771.

    Article  CAS  PubMed  Google Scholar 

  18. S. Gao, H. Miao, X. Tao, B. Jiang, Y. Xiao, F. Cai, Y. Yun, J. Li, and W. Chen, J. Chromatogr. B, 2011, 879, 1939.

    Article  CAS  Google Scholar 

  19. M. Shibata, S. Hashi, H. Nakanishi, S. Masuda, T. Katsura, and I. Yano, Biomed. Chromatogr., 2012, 26, 1519.

    Article  CAS  PubMed  Google Scholar 

  20. X. Zhu, J. Liu, J. Wu, R. Cao, and T. Li, J. Chromatogr. B, 2014, 967, 50.

    Article  CAS  Google Scholar 

  21. K. H. Kim, Y. H. Ahn, E. S. Ji, J. Y. Lee, J. Y. Kim, H. J. An, and J. S. Yoo, Anal. Chim. Acta, 2015, 882, 38.

    Article  CAS  PubMed  Google Scholar 

  22. T. G. Hammond, S. Moes, S. Youhanna, P. Jennings, O. Devuyst, A. Odermatt, and P. Jenö, Bioanalysis, 2016, 8, 1279.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Madoka Ogata, Mizusa Inui, Akihiro Hamana, Manaka Hayashi, Yuki Kamiya, Syunsuke Sakata, and Mari Nagao for their excellent technical assistance. This work was partly supported by a Grant-in-aid for Challenging Exploratory Research (Grant No. 26560072) from the Japan Society for the Promotion of Science (JSPS).

Author information

Authors and Affiliations

  1. Department of Hygienic Sciences, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada, Kobe, 658-8558, Japan

    Maya Kamao, Kimie Nakagawa, Toshio Okano & Hiroshi Hasegawa

  2. Laboratory of Biochemistry, Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma, Saitama, 337-8570, Japan

    Yoshihisa Hirota

  3. Laboratory of Organic Synthesis and Medicinal Chemistry, Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma, Saitama, 337-8570, Japan

    Yoshitomo Suhara

  4. Laboratory of Public Health, Faculty of Health and Nutrition, Osaka Shoin Women’s University, 4-2-26 Hishiya-nishi, Higashiosaka, Osaka, 577-8550, Japan

    Naoko Tsugawa

Authors
  1. Maya Kamao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoshihisa Hirota
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoshitomo Suhara
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Naoko Tsugawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kimie Nakagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Toshio Okano
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hiroshi Hasegawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Maya Kamao or Toshio Okano.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kamao, M., Hirota, Y., Suhara, Y. et al. Determination of Menadione by Liquid Chromatography-Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring. ANAL. SCI. 33, 863–867 (2017). https://doi.org/10.2116/analsci.33.863

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.33.863

Keywords

Search

Navigation