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Characterization of Melanin Radicals in Paraffin-embedded Malignant Melanoma and Nevus Pigmentosus Using X-band EPR and EPR Imaging

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Abstract

Continuous wave electron paramagnetic resonance (CW EPR) and X-band (9 GHz) EPR imaging (EPRI) were used to nondestructively investigate the possible differentiation between malignant melanoma (MM) and nevus pigmentosus (NP) melanin radicals in paraffin-embedded specimens. The EPR spectra of both samples were analyzed using linewidth, spectral pattern, and X-band EPRI. The CW-EPR spectra of the MM showed an additional signal overlap. Eumelaninand pheomelanin-related radicals were observed in the MM specimens. The EPR results revealed that the peak-to-peak linewidths (ΔHpp) of paraffin-embedded MM and NP samples were 0.65 ± 0.01 and 0.69 ± 0.01 mT, respectively. The {iyg}-value was 2.005 for both samples. Moreover, the two-dimensional (2D) EPRI of the MM showed different signal intensities at the different tumor stages, unlike the NP, which displayed fewer variations in signal intensity. Thus, the present results suggest that EPR and 2D EPRI can be useful for characterization of the two melanin radicals in the MM and for determination of their size and concentration.

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Acknowledgments

Part of this research was supported by KAKENHI Grant numbers JP15K12499 and 25282124 from the Japan Society for the Promotion of Science (JSPS) (K. N.).

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Authors and Affiliations

  1. Division of Regional Innovation, Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki, 036-8564, Japan

    Kouichi Nakagawa

  2. Department of Dermatology, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, 036-8562, Japan

    Satoko Minakawa & Daisuke Sawamura

  3. Application Bruker BioSpin K. K., 3-9 Moriya-cho, Kanagawa, Yokohama, 221-0022, Japan

    Hideyuki Hara

Authors
  1. Kouichi Nakagawa
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  2. Satoko Minakawa
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  3. Daisuke Sawamura
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  4. Hideyuki Hara
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Correspondence to Kouichi Nakagawa.

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Nakagawa, K., Minakawa, S., Sawamura, D. et al. Characterization of Melanin Radicals in Paraffin-embedded Malignant Melanoma and Nevus Pigmentosus Using X-band EPR and EPR Imaging. ANAL. SCI. 33, 1357–1361 (2017). https://doi.org/10.2116/analsci.33.1357

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  • DOI: https://doi.org/10.2116/analsci.33.1357

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