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3,3′,5,5′-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays

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Abstract

We report on the colorimetric oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide using horseradish peroxidase on photolithography-fabricated (P-PAD) and wax-printed (W-PAD) paper-based analytical devices. Fabricating PADs via photolithography exposes the hydrophilic areas to polymers (photoresists) and solvents, not only reducing the hydrophilicity, but also affecting the TMB–H2O2 assay system with an unavoidable incomplete elimination of photoresist during fabrication. Detection signals are then observed in the presence of photoresist residues on the P-PAD, even at a blank HRP concentration.

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Acknowledgments

This research was supported by the Urakami Foundation for Food and Food Culture Promotion.

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Authors and Affiliations

  1. Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita, Sapporo, 060-8628, Japan

    Lori Shayne Alamo Busa, Takeshi Komatsu & Saeed Mohammadi

  2. Physical Sciences Department, College of Arts and Sciences, Nueva Vizcaya State University, Bayombong, Nueva Vizcaya, 3700, Philippines

    Lori Shayne Alamo Busa

  3. Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita, Sapporo, 060-8628, Japan

    Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani & Manabu Tokeshi

  4. ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa, Nagoya 464–8603, Japan

    Manabu Tokeshi

  5. Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan

    Manabu Tokeshi

  6. Institute of Innovative for Future Society, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan

    Manabu Tokeshi

Authors
  1. Lori Shayne Alamo Busa
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  2. Takeshi Komatsu
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  3. Saeed Mohammadi
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  4. Masatoshi Maeki
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  5. Akihiko Ishida
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  6. Hirofumi Tani
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  7. Manabu Tokeshi
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Correspondence to Manabu Tokeshi.

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Busa, L.S.A., Komatsu, T., Mohammadi, S. et al. 3,3′,5,5′-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays. ANAL. SCI. 32, 815–818 (2016). https://doi.org/10.2116/analsci.32.815

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  • DOI: https://doi.org/10.2116/analsci.32.815

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