Skip to main content
SpringerLink
Log in

Alternating Current Cloud Point Extraction on a Microfluidic Chip: the Use of Ferrocenyl Surfactants

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Alternating current cloud point extraction (ACPE) is a preconcentration technique that can be employed in the analysis of membrane proteins on a microfluidic chip. However, the selectivity of ACPE relies on the hydrophobicity of the analytes. In this study, 11-ferrocenyltrimethylundecylammonium bromide (FTMA) was utilized to introduce electrostatic interaction as part of the ACPE technique. The use of ACPE with oxidized FTMA resulted in efficient concentration of fluorescently labeled anionic membrane proteins. We expect the approach outlined in this report to be useful in the preconcentration technique of microchip electrophoresis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. T. Culbertson, T. G. Mickleburgh, S. A. Stewart-James, K. A. Sellens, and M. Pressnall, Anal. Chem., 2014, 86, 95.

    Article  CAS  PubMed  Google Scholar 

  2. F. Kitagawa, T. Kawai, K. Sueyoshi, and K. Otsuka, Anal. Sci., 2012, 28, 85.

    Article  CAS  PubMed  Google Scholar 

  3. F. A. Shaikh and V. M. Ugaz, Proc. Natl. Acad. Sci. U. S. A., 2006, 103, 4825.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. J. H. Lee, S. Chung, S. J. Kim, and J. Y. Han, Anal. Chem., 2007, 79, 6868.

    Article  CAS  PubMed  Google Scholar 

  5. K. Sueyoshi, F. Kitagawa, and K. Otsuka, Anal. Sci., 2013, 29, 133.

    Article  CAS  PubMed  Google Scholar 

  6. N. Sasaki, Anal. Sci., 2012, 28, 3.

    Article  CAS  PubMed  Google Scholar 

  7. N. Sasaki, K. Hosokawa, and M. Maeda, Lab Chip, 2009, 9, 1168.

    Article  CAS  PubMed  Google Scholar 

  8. N. Sasaki, A. Takemura, and K. Sato, Electrophoresis, 2012, 33, 3159.

    Article  CAS  PubMed  Google Scholar 

  9. N. Sasaki, C. Maekawa, and K. Sato, Electrophoresis, 2015, 36, 424.

    Article  CAS  PubMed  Google Scholar 

  10. H. Tani, T. Kamidate, and H. Watanabe, Anal. Sci., 1998, 14, 875.

    Article  CAS  Google Scholar 

  11. X. Liu and N. L. Abbott, J. Colloid Interface Sci., 2009, 339, 1.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. C. A. Rosslee and N. L. Abbott, Anal. Chem., 2001, 73, 4808.

    Article  CAS  PubMed  Google Scholar 

  13. Y. Kakizawa, H. Sakai, T. Saji, N. Yoshino, Y. Kondo, and M. Abe, J. Jpn. Soc. Colour Mater., 1999, 72, 78.

    Article  CAS  Google Scholar 

  14. T. Gu and P. A. Galera-Gomez, Colloids Surf, A, 1995, 104, 307.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by the INOUE ENRYO Memorial Foundation for Promoting Sciences.

Author information

Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Science and Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama, 350-8585, Japan

    Yuya Usui & Naoki Sasaki

Authors
  1. Yuya Usui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naoki Sasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Naoki Sasaki.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Usui, Y., Sasaki, N. Alternating Current Cloud Point Extraction on a Microfluidic Chip: the Use of Ferrocenyl Surfactants. ANAL. SCI. 32, 109–111 (2016). https://doi.org/10.2116/analsci.32.109

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.32.109

Keywords

Search

Navigation