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Fabrication and Characterization of a Thermostable Quinoprotein Aldose Sugar Dehydrogenase Immobilized Electrode

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Abstract

We fabricated a thermostable quinoprotein aldose sugar dehydrogenase (tPQQ-ASD derived from a hyperthermophilic archaeon Pyrobaculum aerophilum) immobilized electrode. The electrode was prepared by immobilizing agarose gel mixed with the enzyme and carbon nanofiber (CNF) on a carbon paste (CP) electrode containing p-benzoquinone (BQ) as an electron mediator. The electrocatalytic response was clearly observed by the addition of D-glucose at the electrode. The electrode properties such as pH, temperature dependency and substrate selectivity basically followed the enzyme properties. The current response against D-glucose increased with measurement temperatures up to 70°C, and response perturbation caused by dissolved oxygen level was not observed at the electrode. As for the results of long-term stability evaluation, the current response was stable for 30 days when the electrode was stored in HEPES buffer solution (pH 7.0) at 4°C.

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Authors and Affiliations

  1. Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima, 2-1 Minami-josanjima, Tokushima, 770-8506, Japan

    Yohei Yamada, Tetsuya Hayashi, Tomoki Yabutani & Toshio Takayanagi

  2. Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kita, Kagawa, 761-0795, Japan

    Haruhiko Sakuraba

Authors
  1. Yohei Yamada
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  2. Tetsuya Hayashi
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  3. Haruhiko Sakuraba
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  4. Tomoki Yabutani
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  5. Toshio Takayanagi
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Correspondence to Tomoki Yabutani.

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Yamada, Y., Hayashi, T., Sakuraba, H. et al. Fabrication and Characterization of a Thermostable Quinoprotein Aldose Sugar Dehydrogenase Immobilized Electrode. ANAL. SCI. 29, 79–83 (2013). https://doi.org/10.2116/analsci.29.79

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  • DOI: https://doi.org/10.2116/analsci.29.79

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