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Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

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Abstract

A uric acid biosensor based on the direct electron transfer of a hemoglobin-encapsulated chitosan-modified glassy carbon electrode was developed for a highly sensitive and selective analysis in urine samples. The modified electrode was prepared by the encapsulation of hemoglobin and uricase in a chitosan matrix. The hydrogen peroxide produced from the catalytic oxidation of uric acid by uricase was reduced electrocatalytically by immobilized hemoglobin and used to obtain a sensitive amperometric response to uric acid. The linear response of the uric acid concentrations ranged from 2.00 to 30.0 µM with a correlation of 0.9982, the detection limit of uric acid was estimated to be 0.85 µM at a signal/noise ratio of 3. The uric acid biosensor can efficiently exclude the interference of commonly coexisted ascorbic acid, dopamine, epinephrine, etc. The relative standard deviation was under 2.56% (n = 5) for the determination of real samples. This biosensor is satisfactory for the determination of human urine samples compared with the HPLC-UV method.

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Authors and Affiliations

  1. Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology, 53 Zhengzhou Road, 266042, Qingdao, China

    Changzhi Zhao, Li Wan, Qin Wang, Shufeng Liu & Kui Jiao

Authors
  1. Changzhi Zhao
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  2. Li Wan
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  3. Qin Wang
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  4. Shufeng Liu
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  5. Kui Jiao
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Correspondence to Changzhi Zhao.

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Zhao, C., Wan, L., Wang, Q. et al. Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode. ANAL. SCI. 25, 1013–1017 (2009). https://doi.org/10.2116/analsci.25.1013

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