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Live Single-Cell Molecular Analysis by Video-Mass Spectrometry

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Abstract

The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.

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Authors and Affiliations

  1. Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami, Hiroshima, 734-8553, Japan

    Naohiro Tsuyama, Hajime Mizuno, Emi Tokunaga & Tsutomu Masujima

Authors
  1. Naohiro Tsuyama
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  2. Hajime Mizuno
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  3. Emi Tokunaga
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  4. Tsutomu Masujima
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Correspondence to Tsutomu Masujima.

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Tsuyama, N., Mizuno, H., Tokunaga, E. et al. Live Single-Cell Molecular Analysis by Video-Mass Spectrometry. ANAL. SCI. 24, 559–561 (2008). https://doi.org/10.2116/analsci.24.559

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  • DOI: https://doi.org/10.2116/analsci.24.559

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