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Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol

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Abstract

In this work, an alkaline phosphatase (ALP) biosensor was established based on G-quadruplex/N-methylmesoporphyrin IX (G4/NMM) and p-nitrophenol (PNP). Because the absorption of PNP was close to the excitation wavelength of G4/ NMM, PNP could reduce the fluorescence of G4/NMM. Meanwhile, PNP was the hydrolysis product of p-nitrophenylphosphate (PNPP) by ALP. Therefore, ALP could be detected. This ALP biosensor had a linear analytical range from 2.5 to 25 U/L a the detection limit of 0.81 U/L. Moreover, it showed a satisfactory selectivity and recovery rates.

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Acknowledgments

The work is supported by the National Natural Science Foundation of China (31601536, 31871888) and State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution (GHBK- 2020-001).

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Authors and Affiliations

  1. College of Life Science, Yangtze University, 266 Jingmi Road Jingzhou, Hubei 434025, China

    Liyuan Ma, Yao Xiao, Huajuan Fang, Hualin Yang & Yu Zhou

  2. State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution, Chengdu University of Technology, Chengdu, Sichuan, 610059, China

    Hualin Yang

  3. College of Animal Science, Yangtze University, 266 Jingmi Road Jingzhou, Hubei, 434025, China

    Yu Zhou

Authors
  1. Liyuan Ma
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  2. Yao Xiao
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  3. Huajuan Fang
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  4. Hualin Yang
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  5. Yu Zhou
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Correspondence to Hualin Yang or Yu Zhou.

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Ma, L., Xiao, Y., Fang, H. et al. Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol. ANAL. SCI. 37, 1487–1489 (2021). https://doi.org/10.2116/analsci.21C010

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  • DOI: https://doi.org/10.2116/analsci.21C010

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