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Investigations of Chirality Effects on Undifferentiated State of Mesenchymal Stem Cells Using Soft Nanofibrous Oligopeptide Hydrogels

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Abstract

Soft nanofibrous oligopeptide hydrogels consisting of self-assembled l- and d-form Fmoc-Phe-Phe-Cys networks photo-cross-linked by poly(ethylene glycol)-dimethacrylate, which are referred to as l- and d-gel, respectively, were developed for investigation of chirality effects on the undifferentiated state of mesenchymal stem cells. Encapsulated mesenchymal stem cells in d-gel showed slower growth and less spreading, resulting in higher maintenance of the undifferentiated state, compared to in l-gel. This indicates that d-form peptide materials might be useful as scaffold materials for regenerative medicine using stem cells.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science KAKENHI [grant numbers: 16K16398 and 19H05458 (to T. Y.)].

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Authors and Affiliations

  1. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153-8902, Japan

    Toru Yoshitomi & Keitaro Yoshimoto

  2. Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan

    Toru Yoshitomi

  3. Institute for Frontier Life and Medical Sciences, Kyoto University, Yoshidahonmachi, Sakyo, Kyoto, 606-8507, Japan

    Hangyu Zheng

Authors
  1. Toru Yoshitomi
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  2. Hangyu Zheng
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  3. Keitaro Yoshimoto
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Correspondence to Toru Yoshitomi or Keitaro Yoshimoto.

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Yoshitomi, T., Zheng, H. & Yoshimoto, K. Investigations of Chirality Effects on Undifferentiated State of Mesenchymal Stem Cells Using Soft Nanofibrous Oligopeptide Hydrogels. ANAL. SCI. 37, 539–543 (2021). https://doi.org/10.2116/analsci.20SCN05

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  • DOI: https://doi.org/10.2116/analsci.20SCN05

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