Abstract
The uptake of Cu2+ was investigated using various types of liposomes composed of phosphatidylcholine (PC), cholesterol (Chol) and dicethylphosphate (DCP). DCP played a role as a ligand for Cu2+. Multilamellar vesicles (MLVs) were more effective for the uptake of Cu2+ compared to unilamellar vesicles prepared by the extrusion technique. The uptake efficiency of MLVs for Cu2+ was dependent on the molar ratio of DCP in MLVs. The uptake percent of Cu2+ was 92% using MLVs having a PC:DCP:Chol molar ratio of 4:3:3; 95% of the total vesicle Cu2+ was bound to DCP of the outer membrane surface of the MLVs, and the remaining 5% of the total Cu2+ was distributed into the interior side of the MLVs. MLVs having a PC:DCP:Chol molar ratio of 4:3:3 were also effective as separation media for Mn2+, Co2+, Ni2+ and Zn2+. The uptake efficiency of the MLVs for the transition-metal ions increased in the order Co2+ < Zn2+ < Ni2+ < Mn2+ < Cu2+.
Similar content being viewed by others
References
B. Volesky and Z. R. Holan, Biotechnol. Prog., 1995, 11, 235.
F. Veglio’ and F. Beolchini, Hydrometallurgy, 1997, 44, 301.
J. H. van Zanten and H. G. Monbouquette, Biotechnol. Prog., 1992, 8, 546.
I. Stanish and H. G. Monbouquette, J. Membr. Sci., 2001, 192, 99.
T. Kamidate, J. Yamaguchi, T. Suita, H. Tani, and H. Watanabe, Chem. Lett., 1997, 971.
H. Schott and F. A. A. Sayeed, J. Colloid Interface Sci., 1986, 112, 274.
N. Oku, D. A. Kendall, and R. C. Macdonald, Biochim. Biophys. Acta, 1982, 691, 332.
T. Kamidate, T. Suita, and H. Watanabe, Anal. Biochem., 1996, 241, 264.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kamidate, T., Hashimoto, Y., Tani, H. et al. Uptake of Transition Metal Ions Using Liposomes Containing Dicetylphosphate as a Ligand. ANAL. SCI. 18, 273–276 (2002). https://doi.org/10.2116/analsci.18.273
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.18.273