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Time-Resolved Fluorometric Detection of DNA Using a Tetradentate β-Diketonate Europium Chelate as a Label

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Abstract

A tetradentate β-diketonate europium chelate, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chlorosulfo-o-terphenyl(BHHCT)-Eu3+, was labeled to a streptavidin-bovine serum albumin conjugate for time-resolved fluorometric detection of DNA by hybridization with a biotinylated probe DNA in a microtiter well. The method gave a detection limit of 23 pg/well (solid-phase measurement) and 4 pg/well (solution-phase measurement) for specific DNA.

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Authors and Affiliations

  1. Department of Chemistry, Waseda University, Shinjuku, Tokyo, 169-8555, Japan

    Kenji Yoshikawa, Jingli Yuan & Kazuko Matsumoto

  2. Japan Science and Technology Corporation (JST), Japan

    Jingli Yuan, Kazuko Matsumoto & Hiroko Kimura

  3. Department of Forensic Medicine, Juntendo University School of Medicine, Bunkyo, Tokyo, 113-8421, Japan

    Hiroko Kimura

Authors
  1. Kenji Yoshikawa
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  2. Jingli Yuan
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  3. Kazuko Matsumoto
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  4. Hiroko Kimura
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Yoshikawa, K., Yuan, J., Matsumoto, K. et al. Time-Resolved Fluorometric Detection of DNA Using a Tetradentate β-Diketonate Europium Chelate as a Label. ANAL. SCI. 15, 121–124 (1999). https://doi.org/10.2116/analsci.15.121

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  • DOI: https://doi.org/10.2116/analsci.15.121

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