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Amperometric Alcohol-Sensing Electrode Based on a Polyion Complex Membrane Containing Alcohol Oxidase

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Abstract

An amperometric alcohol-sensing electrode was prepared by immobilizing alcohol oxidase (AOx) in a polyion complex membrane. Three kinds of aqueous solutions (poly(4-styrenesulfonate)-, AOx- and poly-L-lysine-containing solutions) were successively placed on the surface of a glassy carbon electrode and the electrode was allowed to dry. The anodic current (at 1 V vs. Ag/AgCl) of the enzyme electrode increased immediately after the addition of alcohols (e.g., ethanol), and reached a steady state within 5 s. A linear response to ethanol was observed up to 0.4 mM with a detection limit of 0.5 μM. The polyion complex membrane showed permselectivity based on the solute size with a molecule weight cut-off of ca. 100, which was very effective in suppressing the electrochemical interference by L-ascorbic acid and uric acid. The enzyme electrode was applied to the determination of ethanol in alcoholic drinks and soy sources. The electrode could be used for two weeks.

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Authors and Affiliations

  1. National Institute of Bioscience and Human-Technology, Higashi, Tsukuba, Ibaraki, 305, Japan

    Fumio Mizutani, Soichi Yabuki & Seiichiro Iijima

Authors
  1. Fumio Mizutani
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  2. Soichi Yabuki
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  3. Seiichiro Iijima
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Mizutani, F., Yabuki, S. & Iijima, S. Amperometric Alcohol-Sensing Electrode Based on a Polyion Complex Membrane Containing Alcohol Oxidase. ANAL. SCI. 13, 83–87 (1997). https://doi.org/10.2116/analsci.13.83

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  • DOI: https://doi.org/10.2116/analsci.13.83

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