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Poly(N-isopropylacrylamide) Carrying for Affinity Separation of Genotoxins Double-Stranded DNA

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Abstract

We immobilized double-stranded DNA on poly(N-isopropylacrylamide), which is temperature-sensitive and gives precipitates from an aqueous solution when heated over 31°C. Photochemical conjugation with a vinyl derivative of psoralen was demonstrated to make the DNA polymerizable with a vinyl monomer. The radical copolymerization with N-isopropylacrylamide gave the temperature-responsive conjugate. The conjugate was shown to capture ethidium, a DNA-binding genotoxin, and precipitate with it when heated. About 95% of ethidium was separated from its highly diluted solution (3 ppm).

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Authors and Affiliations

  1. Department of Chemical Science and Technology, Faculty of Engineering, Kyushu University, Hakozaki, Fukuoka, 812-81, Japan

    Daisuke Umeno & Mizuo Maeda

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  1. Daisuke Umeno
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  2. Mizuo Maeda
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Umeno, D., Maeda, M. Poly(N-isopropylacrylamide) Carrying for Affinity Separation of Genotoxins Double-Stranded DNA. ANAL. SCI. 13, 553–556 (1997). https://doi.org/10.2116/analsci.13.553

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  • DOI: https://doi.org/10.2116/analsci.13.553

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