Abstract
Endocannabinoid 2-arachidonoylglycerol (2-AG) regulates several important physiological processes in the brain. 2-AG is commonly quantified by gas chromatography mass spectrometry after an initial purification step. The most precise and rapid purification utilizes C18 solid-phase extraction, but quantification problems can arise with acyl migration from 2-AG to 1 -arachidonoylglycerol. We found that extraction with methanol promoted this migration, but acetone and diethyl ether (Et2O) did not. Acetone and Et2O were used to develop a purification method for the direct determination of 2-AG.
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Higuchi, S., Irie, K., Nakano, T. et al. Reducing Acyl Migration during Purification of 2-Arachidonoylglycerol from Biological Samples before Gas Chromatography Mass Spectrometry Analysis. ANAL. SCI. 26, 1199–1202 (2010). https://doi.org/10.2116/analsci.26.1199
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DOI: https://doi.org/10.2116/analsci.26.1199