Effect of hydroxyapatite-coated nanofibrous membrane on the responses of human periodontal ligament fibroblast

Sun-Hye PARK; Tae-Il KIM; Young KU; Chong-Pyoung CHUNG; Soo-Boo HAN; Ji-Hee YU; Seung-Pyo LEE; Hae-Won KIM; Hae-Hyoung LEE

doi:10.2109/jcersj2.116.31

Feature: Ceramics for Biomedical Applications in Asian Countries: Papers

Effect of hydroxyapatite-coated nanofibrous membrane on the responses of human periodontal ligament fibroblast

Sun-Hye PARK, Tae-Il KIM, Young KU, Chong-Pyoung CHUNG, Soo-Boo HAN, Ji-Hee YU, Seung-Pyo LEE, Hae-Won KIM, Hae-Hyoung LEE

Author information

Sun-Hye PARK
Department of Periodontology, Dental Research Institute, School of Dentistry
Tae-Il KIM
Department of Periodontology, Dental Research Institute, School of Dentistry
Young KU
Department of Periodontology, Dental Research Institute, School of Dentistry
Chong-Pyoung CHUNG
Department of Periodontology, Dental Research Institute, School of Dentistry
Soo-Boo HAN
Department of Periodontology, Dental Research Institute, School of Dentistry
Ji-Hee YU
Department of Oral anatomy, School of Dentistry
Seung-Pyo LEE
Department of Oral anatomy, School of Dentistry
Hae-Won KIM
Department of Biomaterials Science & Institute of Tissue Regeneration Engineering, School of Dentistry
Hae-Hyoung LEE
Department of Biomaterials Science & Institute of Tissue Regeneration Engineering, School of Dentistry

Keywords: Biomaterial, Hydroxyapatite, Membrane, Periodontal ligament fibroblast, Periodontal tissue regeneration

JOURNAL FREE ACCESS

2008 Volume 116 Issue 1349 Pages 31-35

DOI https://doi.org/10.2109/jcersj2.116.31

[Correction] J. Ceram. Soc. Japan Vol.116 (2008) No.1351 p.510

Details

Abstract

In this study, hydroxyapatite-coated biopolymer nanofibrous membrane was synthesized by mineralizing the electrospun polycaprolactone nanofiber. The effect of the hydroxyapatite-coated nanofibrous membrane was mainly investigated on the proliferation and differentiation of human periodontal ligament fibroblast, which is necessary for periodontal tissue regeneration. Scanning electron microscopy revealed favorable cell attachment and spreading appearance on 1 d and MTS assay showed increased cell proliferation on the hydroxyapatite-coated nanofiber membrane during 14 d. From 7 to 14 d, alkaline phosphatase activity of the hydroxyapatite-coated nanofiber was significantly increased when compared to that of control group. Hydroxyapatite-coated nanofibrous membrane showed prominent mineral formation on day 14. As a result, it was clarified that the developed hydroxyapatite-coated nanofibrous membrane had favorable effects on the proliferation and differentiation of human periodontal ligament fibroblast and might be a good candidate material for periodontal tissue regeneration.

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