Evaluation of diosgenin content in diferent genotypes of fenugreek
(Trigonella foenum-graecum L.)

Abstract :

Response surface methodology and central composite design were used to optimize a medium for the production of biomass of Streptomyces anulatus and herbicidal metabolites. The four variables involved in growth of the organism were the temperature, pH, galactose and serine concentrations. The optimized medium for the production of biomass contained, galactose -75 mg/ml and serine - 7.5 mg/ml and the temperature - 43oC and pH- 8. This medium resulted in increase in the level of biomass production (1.65g/l) compared to the yield in the normal production medium prior to optimized level (1.01 g/ml) after 120 h of fermentation, whereas the value predicted by the quadratic model was 1.03 g/l .