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Submitted
14 August 2017
Accepted
14 August 2017
Published
15 August 2017
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Abstract

Pain is the most common complaints in everyday practice. Pain is a clinical manifestation caused by stimuli due to potential tissue damage. Low level laser therapy (LLLT) has been used in reducing pain. One of indicator tissue healing is activation of transforming growth factor -b1(TGF-b1). Tissue healing will eliminate the pain. Do LLLT stimulate TGF-b1 expression? The objective of this study was to investigate the expression of TGF-b1 in low level laser therapy. Thirty male Wistar rats, body weight 250 – 300 gram, 3 month old, were divided into 3 groups, negative control (n=10), positive control (n=10) and treatment groups (n=10). The positive control and treatment groups were injected with Complete’s Freund Adjuvant (CFA) 100 mL at left footpad. The treatment group treated with low level laser therapy Lasermed type 2100 wavelength 905 nm, out power 25-500 mW, dose 1 joule/cm2;5 times with interval 24 hours on left footpad. Samples were taken from the skin of footpad and immunohistochemical examination with monoclonal antibody anti- TGF-b1. Calculations carried out on brown reaction in macrophage cell. Data were analyzed using the Games Howell with SPSS 17. There were significant differences in the expression of TGF-b1 in treatment group (15.8±3.1) compared with the positive control group (3.9±1.1) and negative control group (4.6±2.1). There were no significant differences between expression TGF-b1 in positive control group and negative control group. In conclusion, low level laser therapy increased the expression of TGF-b1.

Keywords

Low level laser therapy TGF-1

Article Details

How to Cite
Subadi, I., Wardhani, I. L., & Andriati, A. (2017). THE EXPRESSION OF TGF-1 AFTER LOW LEVEL LASER THERAPY IN INFLAMMATION ANIMAL MODEL. Folia Medica Indonesiana, 53(1), 29–32. https://doi.org/10.20473/fmi.v53i1.5486
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References

  1. Alves ACA, Vieira RP, Leal ECP, Santos SA, Ligeiro AP, Albertini R et al (2013). Effect of low level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation. Arthritis Research & Therapy 15, 1-11.
  2. Amid R, Kadkhodazadeh M, Ahsaie MG and Hakakzdeh A (2014). Effect of low level laser therapy on proliferation and differentiation of the cells contributing in bone regeneration. Journal of Lasers in Medical Sciences 5(4), 163-2014.
  3. Avci P, Gupta A, Sadasivam M, Vecchio D, Pam Z, Pam N & Hamblin MR (2013). Low-Level Laser Therapy (LLLT) in Skin: Stimulating, Healing, Restoring. Seminars in Cutaneous Medicine and Surgery 32, 41-52.
  4. Beckman KH, Meyer-Hamme G, and Schroder S (2014). Low Level Laser Therapy for treatment of Diabetic Foot Ulcers: A critical survey. Evidence-Based Complementary and Alternative Medicine 2014, 1-9.
  5. Breivik H, Collett B, Ventafridda V, Cohen R and Gallacher D (2006). Survey of chronic pain in Europe: Prevalence, impact on daily life, and treatment. European Journal of Pain 10, 287-333.
  6. Buvanendran A and Lipman AG (2010). Nonsteroidal anti-inflammatory drugs and acetaminophen. In: Fishman SM, Ballantyne JC, Rathmell JP (eds). Bonica’s Management of Pain, 4th ed., Philadelphia, Wolter Kluwer – Lippincott Williams and Wilkins, p 1157-1171.
  7. Croft P, Blyth FM and Windt D (2010). The global occurence of chronic pain. In: Croft P, Blyth FM and Windt D (eds). Chronic Pain Epidemiology. New York, Oxford University Press, p 9-18.
  8. Fujimori S, Gudis K. and Sakamoto C (2010). A review of Anti-Inflammatory Drug-Induces Gastrointestinal Injury. Pharmaceuticals 3, 1187-1201.
  9. Hamblin MR and Demidova TN (2006). Mechanism of low level light therapy. Proc.of SPIE 6140, 1-12.
  10. Hegedus B, Viharos L, Gervain M & Galfi M (2009). The Effect of Low-Level Laser in Knee Osteoarthritis: A Double-Blind, Randomized, Placebo-Controlled Trial. Photomedicine and Laser Surgery 27(4), 577-584.
  11. Johannes CB, Lee TK, Zhou X, Johnston JA and Dworkin RH (2010). The Prevalence of Chronic Pain in United States Adult: Result of Internet-Based Survey. The Journal of Pain 11, 1230-1239.
  12. Kajagar BM, Godhi AS, Pandit A, Khatri S (2014). Efficacy of Low Level Laser Therapy on Wound Healing in Patients with Chronic Diabetic Foot Ulcers. Indian Journal Surgery 74 (5), 359-363
  13. Reyes-Gibby C, Torres-Vigil I and Croock R (2008). Epidemiology of chronic pain: classical to molecular approaches to understanding the epidemiology of pain. In: Wilson PR, Watson PJ, Haythornthwaite JA and Jensen TS (eds). Chronic Pain, 2nd ed., London, Hodder Arnold, p 65-75.
  14. Turk DC and Okifuji (2010). Pain term and taxonomies of pain. In: Fishman SM, Ballantyne JC and Rathmell JP (eds). Bonica’s Management of Pain, 4th ed., Philadephia, Lippincott William and Wilkins, p 13-23.
  15. Wong WS and Feilding R (2011). Prevalence and Characteristics of Chronic Pain in the General Population of Hong Kong. The Journal of Pain 12, 236-245.
  16. Zhang RX and Ren K (2011). Animal Models of Inflammatory Pain. In: Ma C and Zhang (eds). Animal Models of Pain. New York, Humana Press, p 23-40.