MECHANISM OF APOPTOSIS INHIBITION TO SQUAMOUS CELL CARCINOMA OF ORAL CANCER IN CISPLATIN TREATMENT

R Marjono Dwi Wibowo; David S Perdanakusuma; Eddy Herman Tanggo

doi:10.20473/fmi.v53i1.5482

Submitted

14 August 2017

Accepted

14 August 2017

Published

15 August 2017

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Abstract

This study was to approve the increased secretion of Hsp 70, DNA damage, and inhibitor apoptosis protein in cisplatin therapy which influence apoptosis of oral cancer cell and to know mechanism of molecular pathology. This study was an in vitro experimental laboratory using Randomized Block Design. Cell culture of oral cancer divided from cisplatin resistance cancer cell and cancer cell never induce cisplatin. Two group of cancer cell would be given cisplatin therapy. Secretion of Hsp 70, DNA damage, Inhibitor of apoptosis protein, and apoptosis index would be examined. Cisplatin resistance cancer cell group showed lower apoptosis than never induce cisplatin cancer cell. Elevated secretion of Hsp 70 in cisplatin therapy group (p= 0.000, b=0.881). Lower secretion of DNA damage protein in cisplatin resistance cancer cell and it was not going apoptosis. In path regression analysis, cisplatin was significans through IAP pathway (p=0.000, b=0.726) to apoptosis. All type of cell cultures were also significans through IAP pathway (p=0.000, b=0.496) to apoptosis. Elevated IAP secretion influenced apoptosis (b= 1.000). In conclusion, cisplatin used IAP pathway to apoptosis. All type of cell cultures also used IAP pathway to apoptosis. Cisplatin resistance cell culture had stronger effect to IAP and IAP increased inhibition to apoptosis.

Keywords

Cisplatin in vitro cell culture Hsp 70 DNA damage IAP apoptosis

How to Cite

Dwi Wibowo, R. M., Perdanakusuma, D. S., & Tanggo, E. H. (2017). MECHANISM OF APOPTOSIS INHIBITION TO SQUAMOUS CELL CARCINOMA OF ORAL CANCER IN CISPLATIN TREATMENT. Folia Medica Indonesiana, 53(1), 1–6. https://doi.org/10.20473/fmi.v53i1.5482

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References

Ambrosini G, Adida C, Altieri DC (1997). A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 3, 917–921
Cepeda V, Fuertes MA, Castilla J, Alonso C, Quevedo C, Pérez JM (2007). Biochemical mechanisms of cisplatin cytotoxicity. Anticancer Agents Med Chem 7, 3-18
Deveraux QL and Reed JC (1999). IAP family proteins-suppressors of apoptosis. Genes Dev 13, 239-252
Duckett CS, Nava VE, Gedrich RW, Clem RJ, Van Dongen JL, Gilfillan MC, Shiels H, Hardwick JM, Thompson CB (1996). A conserved family of cellular genes related to the baculovirus iap gene and encoding apoptosis inhibitors. EMBO J 15, 2685–2694
Forastiere AA, Goepfert H, Maor M, Pajak TF, Weber R, Morrison W, Glisson B, Trotti A, Ridge JA, Chao C, Peters G, Lee DJ, Leaf A, Ensley J, Cooper J (2003). Concurrent chemotherapy and radiotherapy for organ preservation in advanced laryngeal cancer. N Engl J Med 349, 2091-2098
Gober MD, Wales SQ, Aurelian L (2005). Herpes simplex virus type 2 encodes a heat shock protein homologue with apoptosis regulatory functions. Front Biosci 10, 2788-2803
Gosselin NH, Brunet RC, Carrier G (2003). Comparative occupational exposures to formaldehyde released from inhaled wood product dusts versus that in vapor form. Appl Occup Environ Hyg 18, 384–393
Kelland L (2007). The resurgence of platinum-based cancer chemotherapy. Nat Rev Cancer 7, 573-584
Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC (1998). Control of apoptosis and mitotic spindle checkpoint by survivin. Nature 396, 580–584
Lippard SJ (1987). Chemistry and molecular biology of platinum anticancer drugs. Pure and Applied Chemistry 59, 731–742
Liston P, Roy N, Tamai K, Lefebvre C, Baird S, Cherton-Horvat G, Farahani R, McLean M, Ikeda JE, MacKenzie A, Korneluk RG (1996). Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes. Nature 379, 349–353
Mosser DD and Morimoto RI (2004). Molecular chaperones and the stress of oncogenesis. Oncogene 23, 2907-2918
Roy MD (2007). Approach for assessing total cellular DNA damage. Biotechniques 42, 425-429
Siddik ZH (2003). Cisplatin: mode of cytotoxic action and molecular basis of resistance. Oncogene 22, 7265-7279
Sunters A (2013). How can I create a resistant cell line? Available from https://www.researchgate.net/post/ How_can_I_create_a_resistant_cell_line.Accessed December 15, 2015

References

Ambrosini G, Adida C, Altieri DC (1997). A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 3, 917–921

Cepeda V, Fuertes MA, Castilla J, Alonso C, Quevedo C, Pérez JM (2007). Biochemical mechanisms of cisplatin cytotoxicity. Anticancer Agents Med Chem 7, 3-18

Deveraux QL and Reed JC (1999). IAP family proteins-suppressors of apoptosis. Genes Dev 13, 239-252

Duckett CS, Nava VE, Gedrich RW, Clem RJ, Van Dongen JL, Gilfillan MC, Shiels H, Hardwick JM, Thompson CB (1996). A conserved family of cellular genes related to the baculovirus iap gene and encoding apoptosis inhibitors. EMBO J 15, 2685–2694

Forastiere AA, Goepfert H, Maor M, Pajak TF, Weber R, Morrison W, Glisson B, Trotti A, Ridge JA, Chao C, Peters G, Lee DJ, Leaf A, Ensley J, Cooper J (2003). Concurrent chemotherapy and radiotherapy for organ preservation in advanced laryngeal cancer. N Engl J Med 349, 2091-2098

Gober MD, Wales SQ, Aurelian L (2005). Herpes simplex virus type 2 encodes a heat shock protein homologue with apoptosis regulatory functions. Front Biosci 10, 2788-2803

Gosselin NH, Brunet RC, Carrier G (2003). Comparative occupational exposures to formaldehyde released from inhaled wood product dusts versus that in vapor form. Appl Occup Environ Hyg 18, 384–393

Kelland L (2007). The resurgence of platinum-based cancer chemotherapy. Nat Rev Cancer 7, 573-584

Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC (1998). Control of apoptosis and mitotic spindle checkpoint by survivin. Nature 396, 580–584

Lippard SJ (1987). Chemistry and molecular biology of platinum anticancer drugs. Pure and Applied Chemistry 59, 731–742

Liston P, Roy N, Tamai K, Lefebvre C, Baird S, Cherton-Horvat G, Farahani R, McLean M, Ikeda JE, MacKenzie A, Korneluk RG (1996). Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes. Nature 379, 349–353

Mosser DD and Morimoto RI (2004). Molecular chaperones and the stress of oncogenesis. Oncogene 23, 2907-2918

Roy MD (2007). Approach for assessing total cellular DNA damage. Biotechniques 42, 425-429

Siddik ZH (2003). Cisplatin: mode of cytotoxic action and molecular basis of resistance. Oncogene 22, 7265-7279

Sunters A (2013). How can I create a resistant cell line? Available from https://www.researchgate.net/post/ How_can_I_create_a_resistant_cell_line.Accessed December 15, 2015

MECHANISM OF APOPTOSIS INHIBITION TO SQUAMOUS CELL CARCINOMA OF ORAL CANCER IN CISPLATIN TREATMENT

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