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The Role of Hypericum Perforatum (St. John's Wort Oil) and Olive Oil in the Prevention of Peritoneal Adhesion in a Rat Model: An Animal Study

Year 2022, Volume: 8 Issue: 1, 23 - 30, 25.02.2022
https://doi.org/10.19127/mbsjohs.1008275

Abstract

Objective: We aimed to investigate Hypericum Perforatum (St. John's Wort Oil) and pure olive oil with respect to their effect on preventing intra-abdominal adhesions after abdominal surgery.
Methods: This was an animal study carried out by the Giresun Faculty of Medicine, Department of General Surgery between August 10, 2020, and September 10, 2020. In the study, intra-abdominal adhesion development was examined macroscopically and microscopically on the 14th day after experimental abdominal surgery in three groups [(i) Control, (ii) olive oil application, (iii) St. John's Wort oil application] each consisting of 7 female Wistar Albino rats. Majuzi classification and Zuhlke's microscopic adhesion classification was used to evaluate adhesions.
Results: Macroscopically, fibrous bands were not observed in 1 rat in the control group, 2 rats in olive oil recipients, and 1 rat in St. John's Wort oil recipients. A significant difference between the groups was identified in terms of Zuhlke's microscopic adhesion scores (p = 0.026). Accordingly, the adhesion level was significantly reduced in olive oil recipients compared to controls (p = 0.002). There was no significant difference in terms of adhesion between all groups (p>0.05). The Majuzi adhesion classifications in the control group, olive oil group and St. John's wort groups were also similar (p = 0.308).
Conclusion: In rats who underwent abdominal surgery, it was determined that intra-abdominal olive oil application reduced the level of adhesion, while St. John's Wort oil application had no effect on adhesion formation.

References

  • 1. Dubuisson J, Botchorishvili R, Perrette S, Bourdel N, Jardon K, Rabischong B, et al. Incidence of intraabdominal adhesions in a continuous series of 1000 laparoscopic procedures. Am J Obstet Gynecol. 2010;203(2):111.e1-3.
  • 2. Tabibian N, Swehli E, Boyd A, Umbreen A, Tabibian JH. Abdominal adhesions: A practical review of an often overlooked entity. Ann Med Surg (Lond). 2017;15:9-13.
  • 3. Bruggmann D, Tchartchian G, Wallwiener M, Munstedt K, Tinneberg HR, Hackethal A. Intra-abdominal adhesions: definition, origin, significance in surgical practice, and treatment options. Dtsch Arztebl Int. 2010;107(44):769-75.
  • 4. Uslu Yuvaci H, Cevrioglu AS, Gunduz Y, Akdemir N, Karacan A, Erkorkmaz Ü, et al. Does applied ultrasound prior to laparoscopy predict the existence of intra-abdominal adhesions? Turk J Med Sci. 2020;50(2):304-11.
  • 5. Maciver AH, McCall M, James Shapiro AM. Intra-abdominal adhesions: cellular mechanisms and strategies for prevention. Int J Surg. 2011;9(8):589-94.
  • 6. Arung W, Meurisse M, Detry O. Pathophysiology and prevention of postoperative peritoneal adhesions. World J Gastroenterol. 2011;17(41):4545-53.
  • 7. Urkan M, Özerhan İ H, Ünlu A, Can MF, Özturk E, Gunal A, et al. Prevention of Intraabdominal Adhesions: An Experimental Study Using Mitomycin-C and 4% Icodextrin. Balkan Med J. 2017;34(1):35-40.
  • 8. Oh J, Kuan KG, Tiong LU, Trochsler MI, Jay G, Schmidt TA, et al. Recombinant human lubricin for prevention of postoperative intra-abdominal adhesions in a rat model. J Surg Res. 2017;208:20-5.
  • 9. Ng QX, Venkatanarayanan N, Ho CY. Clinical use of Hypericum perforatum (St John's wort) in depression: A meta-analysis. J Affect Disord. 2017;210:211-21.
  • 10. Huang N, Rizshsky L, Hauck C, Nikolau BJ, Murphy PA, Birt DF. Identification of anti-inflammatory constituents in Hypericum perforatum and Hypericum gentianoides extracts using RAW 264.7 mouse macrophages. Phytochemistry. 2011;72(16):2015-23.
  • 11. Suntar I, Oyardı O, Akkol EK, Ozcelik B. Antimicrobial effect of the extracts from Hypericum perforatum against oral bacteria and biofilm formation. Pharm Biol. 2016;54(6):1065-70.
  • 12. Saddiqe Z, Naeem I, Maimoona A. A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol. 2010;131(3):511-21.
  • 13. Russo E, Scicchitano F, Whalley BJ, Mazzitello C, Ciriaco M, Esposito S, et al. Hypericum perforatum: pharmacokinetic, mechanism of action, tolerability, and clinical drug-drug interactions. Phytother Res. 2014;28(5):643-55.
  • 14. Hajhashemi M, Ghanbari Z, Movahedi M, Rafieian M, Keivani A, Haghollahi F. The effect of Achillea millefolium and Hypericum perforatum ointments on episiotomy wound healing in primiparous women. J Matern Fetal Neonatal Med. 2018;31(1):63-9.
  • 15. Schwingshackl L, Christoph M, Hoffmann G. Effects of Olive Oil on Markers of Inflammation and Endothelial Function-A Systematic Review and Meta-Analysis. Nutrients. 2015;7(9):7651-75.
  • 16. Gavahian M, Khaneghah AM, Lorenzo JM, Munekata PE, Garcia-Mantrana I, Collado MC, et al. Health benefits of olive oil and its components: Impacts on gut microbiota antioxidant activities, and prevention of noncommunicable diseases. Trends Food Sci Technol. 2019;88:220-7.
  • 17. Cicerale S, Lucas LJ, Keast RS. Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Curr Opin Biotechnol. 2012;23(2):129-35.
  • 18. Yildiz T, Ilce Z, Yildirim M, Akdogan M, Yurumez Y, Varlikli O, et al. Antienflamatuar and antiadhesive effect of clioquinol. Int J Surg. 2015;15:17-22.
  • 19. Bayhan Z, Zeren S, Kocak FE, Kocak C, Akcılar R, Kargı E, et al. Antiadhesive and anti-inflammatory effects of pirfenidone in postoperative intra-abdominal adhesion in an experimental rat model. J Surg Res. 2016;201(2):348-55.
  • 20. Sosa S, Pace R, Bornancin A, Morazzoni P, Riva A, Tubaro A, et al. Topical anti-inflammatory activity of extracts and compounds from Hypericum perforatum L. J Pharm Pharmacol. 2007;59(5):703-9.
  • 21. Cakir A, Mavi A, Yildirim A, Duru ME, Harmandar M, Kazaz C. Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L. by activity-guided fractionation. J Ethnopharmacol. 2003;87(1):73-83.
  • 22. Hizli D, Hizli F, Kosus A, Yilmaz S, Kosus N, Haltas H, et al. Effect of Hypericum perforatum on intraperitoneal adhesion formation in rats. Arch Med Sci. 2014;10(2):396-400.
  • 23. Schanuel FS, Saguie BO, Monte-Alto-Costa A. Olive oil promotes wound healing of mice pressure injuries through NOS-2 and Nrf2. Appl Physiol Nutr Metab. 2019;44(11):1199-208.
  • 24. Ural DA, Saruhan H, Saygın I, Aykan DA, Ural A, Imamoglu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. 2019;3(3):218-22.
  • 25. de la Puerta R, Martínez-Domínguez E, Ruíz-Gutiérrez V. Effect of minor components of virgin olive oil on topical antiinflammatory assays. Z Naturforsch C J Biosci. 2000;55(9-10):814-9

The Role of Hypericum Perforatum (St. John's Wort Oil) and Olive Oil in the Prevention of Peritoneal Adhesion in a Rat Model: An Animal Study

Year 2022, Volume: 8 Issue: 1, 23 - 30, 25.02.2022
https://doi.org/10.19127/mbsjohs.1008275

Abstract

Objective: We aimed to investigate Hypericum Perforatum (St. John's Wort Oil) and pure olive oil with respect to their effect on preventing intra-abdominal adhesions after abdominal surgery.
Methods: This was an animal study carried out by the Giresun Faculty of Medicine, Department of General Surgery between August 10, 2020, and September 10, 2020. In the study, intra-abdominal adhesion development was examined macroscopically and microscopically on the 14th day after experimental abdominal surgery in three groups [(i) Control, (ii) olive oil application, (iii) St. John's Wort oil application] each consisting of 7 female Wistar Albino rats. Majuzi classification and Zuhlke's microscopic adhesion classification was used to evaluate adhesions.
Results: Macroscopically, fibrous bands were not observed in 1 rat in the control group, 2 rats in olive oil recipients, and 1 rat in St. John's Wort oil recipients. A significant difference between the groups was identified in terms of Zuhlke's microscopic adhesion scores (p = 0.026). Accordingly, the adhesion level was significantly reduced in olive oil recipients compared to controls (p = 0.002). There was no significant difference in terms of adhesion between all groups (p>0.05). The Majuzi adhesion classifications in the control group, olive oil group and St. John's wort groups were also similar (p = 0.308).
Conclusion: In rats who underwent abdominal surgery, it was determined that intra-abdominal olive oil application reduced the level of adhesion, while St. John's Wort oil application had no effect on adhesion formation.

References

  • 1. Dubuisson J, Botchorishvili R, Perrette S, Bourdel N, Jardon K, Rabischong B, et al. Incidence of intraabdominal adhesions in a continuous series of 1000 laparoscopic procedures. Am J Obstet Gynecol. 2010;203(2):111.e1-3.
  • 2. Tabibian N, Swehli E, Boyd A, Umbreen A, Tabibian JH. Abdominal adhesions: A practical review of an often overlooked entity. Ann Med Surg (Lond). 2017;15:9-13.
  • 3. Bruggmann D, Tchartchian G, Wallwiener M, Munstedt K, Tinneberg HR, Hackethal A. Intra-abdominal adhesions: definition, origin, significance in surgical practice, and treatment options. Dtsch Arztebl Int. 2010;107(44):769-75.
  • 4. Uslu Yuvaci H, Cevrioglu AS, Gunduz Y, Akdemir N, Karacan A, Erkorkmaz Ü, et al. Does applied ultrasound prior to laparoscopy predict the existence of intra-abdominal adhesions? Turk J Med Sci. 2020;50(2):304-11.
  • 5. Maciver AH, McCall M, James Shapiro AM. Intra-abdominal adhesions: cellular mechanisms and strategies for prevention. Int J Surg. 2011;9(8):589-94.
  • 6. Arung W, Meurisse M, Detry O. Pathophysiology and prevention of postoperative peritoneal adhesions. World J Gastroenterol. 2011;17(41):4545-53.
  • 7. Urkan M, Özerhan İ H, Ünlu A, Can MF, Özturk E, Gunal A, et al. Prevention of Intraabdominal Adhesions: An Experimental Study Using Mitomycin-C and 4% Icodextrin. Balkan Med J. 2017;34(1):35-40.
  • 8. Oh J, Kuan KG, Tiong LU, Trochsler MI, Jay G, Schmidt TA, et al. Recombinant human lubricin for prevention of postoperative intra-abdominal adhesions in a rat model. J Surg Res. 2017;208:20-5.
  • 9. Ng QX, Venkatanarayanan N, Ho CY. Clinical use of Hypericum perforatum (St John's wort) in depression: A meta-analysis. J Affect Disord. 2017;210:211-21.
  • 10. Huang N, Rizshsky L, Hauck C, Nikolau BJ, Murphy PA, Birt DF. Identification of anti-inflammatory constituents in Hypericum perforatum and Hypericum gentianoides extracts using RAW 264.7 mouse macrophages. Phytochemistry. 2011;72(16):2015-23.
  • 11. Suntar I, Oyardı O, Akkol EK, Ozcelik B. Antimicrobial effect of the extracts from Hypericum perforatum against oral bacteria and biofilm formation. Pharm Biol. 2016;54(6):1065-70.
  • 12. Saddiqe Z, Naeem I, Maimoona A. A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol. 2010;131(3):511-21.
  • 13. Russo E, Scicchitano F, Whalley BJ, Mazzitello C, Ciriaco M, Esposito S, et al. Hypericum perforatum: pharmacokinetic, mechanism of action, tolerability, and clinical drug-drug interactions. Phytother Res. 2014;28(5):643-55.
  • 14. Hajhashemi M, Ghanbari Z, Movahedi M, Rafieian M, Keivani A, Haghollahi F. The effect of Achillea millefolium and Hypericum perforatum ointments on episiotomy wound healing in primiparous women. J Matern Fetal Neonatal Med. 2018;31(1):63-9.
  • 15. Schwingshackl L, Christoph M, Hoffmann G. Effects of Olive Oil on Markers of Inflammation and Endothelial Function-A Systematic Review and Meta-Analysis. Nutrients. 2015;7(9):7651-75.
  • 16. Gavahian M, Khaneghah AM, Lorenzo JM, Munekata PE, Garcia-Mantrana I, Collado MC, et al. Health benefits of olive oil and its components: Impacts on gut microbiota antioxidant activities, and prevention of noncommunicable diseases. Trends Food Sci Technol. 2019;88:220-7.
  • 17. Cicerale S, Lucas LJ, Keast RS. Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Curr Opin Biotechnol. 2012;23(2):129-35.
  • 18. Yildiz T, Ilce Z, Yildirim M, Akdogan M, Yurumez Y, Varlikli O, et al. Antienflamatuar and antiadhesive effect of clioquinol. Int J Surg. 2015;15:17-22.
  • 19. Bayhan Z, Zeren S, Kocak FE, Kocak C, Akcılar R, Kargı E, et al. Antiadhesive and anti-inflammatory effects of pirfenidone in postoperative intra-abdominal adhesion in an experimental rat model. J Surg Res. 2016;201(2):348-55.
  • 20. Sosa S, Pace R, Bornancin A, Morazzoni P, Riva A, Tubaro A, et al. Topical anti-inflammatory activity of extracts and compounds from Hypericum perforatum L. J Pharm Pharmacol. 2007;59(5):703-9.
  • 21. Cakir A, Mavi A, Yildirim A, Duru ME, Harmandar M, Kazaz C. Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L. by activity-guided fractionation. J Ethnopharmacol. 2003;87(1):73-83.
  • 22. Hizli D, Hizli F, Kosus A, Yilmaz S, Kosus N, Haltas H, et al. Effect of Hypericum perforatum on intraperitoneal adhesion formation in rats. Arch Med Sci. 2014;10(2):396-400.
  • 23. Schanuel FS, Saguie BO, Monte-Alto-Costa A. Olive oil promotes wound healing of mice pressure injuries through NOS-2 and Nrf2. Appl Physiol Nutr Metab. 2019;44(11):1199-208.
  • 24. Ural DA, Saruhan H, Saygın I, Aykan DA, Ural A, Imamoglu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. 2019;3(3):218-22.
  • 25. de la Puerta R, Martínez-Domínguez E, Ruíz-Gutiérrez V. Effect of minor components of virgin olive oil on topical antiinflammatory assays. Z Naturforsch C J Biosci. 2000;55(9-10):814-9
There are 25 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research articles
Authors

Tuğrul Kesicioğlu 0000-0002-9263-5032

İsmail Aydın 0000-0002-9177-6298

Selahattin Vural 0000-0003-1706-3799

İlkay Çinar 0000-0002-2632-3209

Mehmet Gülmez 0000-0001-8803-6160

Arif Keskin 0000-0002-1634-1091

Publication Date February 25, 2022
Published in Issue Year 2022 Volume: 8 Issue: 1

Cite

Vancouver Kesicioğlu T, Aydın İ, Vural S, Çinar İ, Gülmez M, Keskin A. The Role of Hypericum Perforatum (St. John’s Wort Oil) and Olive Oil in the Prevention of Peritoneal Adhesion in a Rat Model: An Animal Study. Mid Blac Sea J Health Sci. 2022;8(1):23-30.

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