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Khirurgii

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Year 2020 Vol. 28 No 6

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

V.Â. MAKAROV 1, N.V. DEDUKH 2, O.À. NIKOLCHENKO 3, V.YE. STREL’NITSKIJ 4, V.V. VASILYEV 4

OSSEOINTEGRATION OF IMPLANTS WITH A DIAMOND-LIKE CARBON COATING IN THE EXPERIMENT

City Clinical Hospital No16 1, Dnepr, Ukraine
D.F. Chebotarev Institute of Gerontology of the National Academy of Medical Sciences of Ukraine2, Kiev, Ukraine
M.I. Sitenko Institute of Spine and Joint Pathologyof theNationalAcademy of Medical Sciences of Ukraine3, Kharkiv, Ukraine
Kharkov Institute of Physics andTechnology4, Kharkiv,
Ukraine

Objective. To study in vitro the integration of the metal stainless steel implants with diamond-like carbon (DLC) coating within living bone of rats.
Methods. The experiment was carried out on albino male rats (n=48; 6 months of age). The steel implants and steel implants with DLC-coating were implanted in the metaphyseal defects of the femur (diameter 2 mm and depth 3 mm)) of the animals in the control and experimental groups (n=24 per group). DLC film was deposited using a filtered vacuum arc plasma flows to the steel implant surface. The coating material was free of metal and hydrogen impurities. The nanohardness of the coating deposited on the sample was from 30 to 40 GPa. The thickness of DLC coating was at least 1 μm. Euthanasia of rats was performed on the 7th, 14th, 30th, and 90th days after surgery by administering a lethal dose of sodium thiopental (90 mg/kg intramuscularly). Bone remodeling around implants was investigated histologically with morphometric assessment of osseointegration (percentage of bone-implant contact).
Results. Diamond-like carbon coating on the surface of steel implants has high adhesive qualities, as evidenced by the attachment of cells to the surface within 7 days after implantation and the formation of bone tissue around them in 14 days.
The formation of a connective tissue with the areas of bone tissue around the steel implants was registered. It was found that for the entire period of the study the osseointegration percentage around the implants made of steel with DLC coating was higher compared to the uncoated implants: by 14 days – 2.6 folds, by 30 days – 2.1 folds, by 90 days – 1.5 folds.
Conclusion. Diamond-like carbon coatings on steel implants are a promising material for use in orthopedics and traumatology due to biocompatibility, osseointegration and the absence of negative remodeling of adjacent bone tissue.

Keywords: diamond-like carbon coating (DLC), bone, osseointegration, light microscopy
p. 617-624 of the original issue
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Address for correspondence:
49000, Ukraine, Dnepr,
Bohdan Khmelnytsky Ave, 19,
City Clinical Hospital No16,
the Combined Trauma Unit,
tel. +3 8099 773-78-38,
e-mail: vasylmakarov2010@gmail.com,
Makarov Vasyl Â.
Information about the authors:
Makarov Vasyl Â., PhD, Orthopedic Surgeon, the Combined Trauma Unit, City Clinical Hospital No16, Dnepr, Ukraine.
https://orcid.org/0000-0003-0936-7039
Dedukh Ninel V., DSBiol, Professor, the Department of Clinical Physiology and Pathology of the Musculoskeletal System, D.F. Chebotarev Institute of Gerontology of the National Academy of Medical Sciences of Ukraine, Kiev, Ukraine.
https://orcid.org/0000-0003-0307-2328
Nikolchenko Olga À., PhD, Senior Researcher, the Department of the Transplantology and Experimental Modeling with an Experimental Biological Clinic, M.I. Sitenko Institute of Spine and Joint Pathologyof the National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine.
https://orcid.org/0000-0001-9808-9485
Strel’nitskij Vladimir Ye., DScPhysic.-Math., Head of the Laboratory of Superhard Amorphous Diamond-Like and Polycrystalline Diamond Coatings, Kharkiv Institute of Physics and Technology, Kharkiv, Ukraine.
https://orcid.org/0000-0001-6047-6981
Vasilyev Vladimir V., PhDTech Sciences, Senior Researcher of the Laboratory of Superhard Amorphous Diamond-Like and Polycrystalline Diamond Coatings, Kharkiv Institute of Physics and Technology, Kharkiv, Ukraine.
https://orcid.org/0000-0002-5705-606X
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