ISHS Acta Horticulturae 567: IV International Strawberry Symposium
GENETIC ENGINEERING OF PARTHENOCARPIC FRUIT DEVELOPMENT IN STRAWBERRY |
| Authors: | B. Mezzetti, L. Landi, L. Scortichini, A. Spena, T. Pandolfini, A. Rebori |
| Keywords: | Strawberry, auxin, parthenocarpy, fruit development, genetic modification |
| DOI: | 10.17660/ActaHortic.2002.567.12 |
| Abstract:
Auxin produced by fertilised ovules is essential for the growth of strawberry fruits. Thus, recombinant DNA methods developed for genetic engineering of parthenocarpy can also be used to study, both in pollinated and parthenocarpic fruits, the role of auxin in controlling strawberry fruit development and quality. Consequently, the parthenocarpic chimeric gene (DefH9-iaaM) has been introduced by Agrobacterium tumefaciens mediated-transformation in the genome of: i) Fragaria vesca, clone Alpina W. Original (2x); ii) F. moschata cultivar ‘Capron Royal’ (6x); iii) F. x ananassa Duch. (8x), selection 93.231.53 of cultivated strawberry. For all the three species, several transgenic clones were isolated and tested on selective proliferation and rooting medium. Afterwards, strawberry transgenic clones were analysed by Southern blot to check the transgenic state and copy number. The development of parthenocarpic achenes in the absence of fertilisation has been conferred by the DefH9-iaaM gene to the three strawberry species. Furthermore, DefH9-iaaM transgenic plants of F. vesca showed an increase in fruit size ranging from 20% to more than 100%, depending on the transgenic event/clone analysed. | |
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