ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
STRUCTURATION OF GENETIC DIVERSITY IN CULTIVATED AND WILD CHERRY TREES USING AFLP MARKERS |
| Authors: | M. Tavaud, A. Zanetto, F. Santi, E. Dirlewanger |
| Keywords: | Prunus avium, sweet cherry tree, mazzard, AFLP (Amplified Fragment Length Polymorphism), genetic diversity, cluster analysis |
| DOI: | 10.17660/ActaHortic.2001.546.32 |
| Abstract:
The success of a breeding program depends on the availability of a diverse germplasm. Therefore, the characterization of germplasm genetic diversity is necessary. In our work, the genetic diversity was analyzed in 63 French cultivated and 165 wild cherry trees (Prunus avium) using AFLP markers. Four primer combinations, chosen among 8 previously tested, allowed us to define 76 markers. A distance matrix was calculated using Jaccard’s coefficient and dendrograms were generated using UPGMA and complete linkage clustering methods. UPGMA cluster analysis separate cultivated and wild cherry trees. Four clusters were identified: 3 for cultivated and 1 for wild trees. Complete linkage cluster analysis lead to the segregation of 5 clusters: three clusters contain mainly cultivated cherry trees whereas two clusters are constituted by wild cherry trees. Initially classified in the Prunus avium species, the 4 varieties of cluster A and 1 using both clustering methods, were previously found in the Prunus cerasus group. In the present study, the isolated position of these clusters could confirm the hypothesis that these varieties might belong to the Prunus cerasus species. Two couples of clusters, including respectively wild and cultivated cherry trees, are closely related. This could suggest at least two different origins of French Prunus avium trees, both cultivated and wild. Lately, the genetic structuration of Prunus avium will be completed by using microsatellite markers and additional genotypes. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
