Screening of Beta-lactam Acylase Producers from Soil and Characterization of Isolates for Substrate Specificity for Cephalosporins

Trupti K  Ashar; V  W  Rajasekar  and Anupama Datla

doi:10.17485/ijst/2015/v8i14/70693

Article

Screening of Beta-lactam Acylase Producers from Soil and Characterization of Isolates for Substrate Specificity for Cephalosporins

VIEWS 705
PDF 230

Abstract
Full-Text HTML
Full-Text PDF
How to Cite

Indian Journal of Science and Technology

DOI: 10.17485/ijst/2015/v8i14/70693

Year: 2015, Volume: 8, Issue: 14, Pages: 1-7

Original Article

Screening of Beta-lactam Acylase Producers from Soil and Characterization of Isolates for Substrate Specificity for Cephalosporins

Trupti K. Ashar^1*, V. W. Rajasekar² and Anupama Datla²

¹Department of Management, AMET University, Chennai, India; [email protected]

²R&D Biotech Division, Fermenta Biotech Ltd, DIL Complex, Majiwada, Thane, 400610, India; [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Beta-lactam acylase enzyme producers were isolated from soil samples. Soil samples were induced with phenyl amino acid derivatives for selelctive isolation. Out of the two potential isolates obtained, one of the isolates was studied for presence of beta-lactam acylase enzyme with cephalexin as model substrate. Enzyme activity of isolate grown in media at varying pH showed it to be tolerant to alkaline pH 8.5. Also the substrate specificity for various cephalosporins showed it most selective to cephalexin.

Keywords: Betalactam Acylase Enzyme, Cephalexin, Soil Isolate