MicroRNA-466 (miR-466) functions as a tumor suppressor and prognostic factor in colorectal cancer (CRC)

Authors

  • Feng Tong Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
  • Youhua Ying Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
  • Haihua Pan Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
  • Wei Zhao Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
  • Hongchen Li Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
  • Xiaoli Zhan Department of Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang, China

DOI:

https://doi.org/10.17305/bjbms.2018.2376

Keywords:

miR-466, colorectal cancer, CRC, prognosis, proliferation, migration, invasion

Abstract

MicroRNAs (miRNAs) have an important role in the regulation of tumor development and metastasis. In this study, we investigated the clinical and prognostic value as well as biological function of miR-466 in colorectal cancer (CRC). Tumor and adjacent healthy tissues were obtained from 100 patients diagnosed with CRC. miR-466 expression was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). mRNA and protein levels of cyclin D1, apoptosis regulator BAX (BAX), and matrix metalloproteinase-2 (MMP-2) were analyzed by qRT-PCR and Western blot, respectively, in SW-620 CRC cells transfected with miR-466 mimics or negative control miRNA. Effects of miR-466 on SW-620 cell proliferation, cell cycle and apoptosis, and invasion were investigated using CCK-8 assay, flow cytometry and Transwell assay, respectively. miR-466 expression was significantly downregulated in tumor tissues compared to matched adjacent non-tumor tissues. Low expression of miR-466 was significantly correlated with the tumor size, Tumor Node Metastasis stage, lymph node metastasis, and distant metastasis. The overall survival of CRC patients with low miR-466 expression was significantly shorter compared to high-miR-466 expression group (log-rank test: p = 0.0103). Multivariate analysis revealed that low miR-466 expression was associated with poor prognosis in CRC patients. The ectopic expression of miR-466 suppressed cell proliferation and migration/invasion, as well as induced G0/G1 arrest and apoptosis in SW-620 cells. Moreover, the ectopic expression of miR-466 decreased the expression of cyclin D1 and MMP-2, but increased BAX expression in SW-620 cells. In conclusion, our findings demonstrated that miR-466 functions as a suppressor miRNA in CRC and may be used as a prognostic factor in these patients.

Author Biographies

  • Feng Tong, Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
    Department of Surgery
  • Youhua Ying, Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
    Department of Surgery
  • Haihua Pan, Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
    Department of Surgery
  • Wei Zhao, Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
    Department of Surgery
  • Hongchen Li, Department of Surgery, Lanxi People’s Hospital, Zhejiang, China
    Department of Surgery
  • Xiaoli Zhan, Department of Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang, China
    Department of Surgery

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MicroRNA-466 (miR-466) functions as a tumor suppressor and prognostic factor in colorectal cancer (CRC)

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Published

01-08-2018

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1.
MicroRNA-466 (miR-466) functions as a tumor suppressor and prognostic factor in colorectal cancer (CRC). Biomol Biomed [Internet]. 2018 Aug. 1 [cited 2024 Mar. 29];18(3):252-9. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/2376