Czech J. Food Sci., 2001, 19(1):8-12 | DOI: 10.17221/6567-CJFS

Determination of aflatoxins in food products by the ELISA method

J. Leszczyñska, J. Mas³owska, A. Owczarek, U. Kucharska
Technical University of Lód¾ - Bioinorganic and Analytical Chemistry Group, Institute of General Food Chemistry, Lód¾, Poland

To determine the total content of aflatoxins, aflatoxin B1 and aflatoxin M1 in food the ELISA method was used. Milk, dairy products and cereal samples were mainly investigated. A few samples were found to be contaminated with aflatoxins. A great usability of the ELISA method for aflatoxin determination in food was established. Selectivity and sensitivity of the method is reported.

Keywords: aflatoxins; ELISA; immunoenzymatic method

Published: February 28, 2001  Show citation

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Leszczyñska J, Mas³owska J, Owczarek A, Kucharska U. Determination of aflatoxins in food products by the ELISA method. Czech J. Food Sci.. 2001;19(1):8-12. doi: 10.17221/6567-CJFS.
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    References

    1. ABOUZIED M.M., AZCONA J.I., BRASELTON W.E., PESTKA J.J. (1991): Immunochemical assessment of mycotoxins. Appl. Environ. Microbiol., 57, 672–675. Go to original source... Go to PubMed...
    2. BARBIERI G., BERGAMINI C., ORI E., RESCA P. (1994): Aflatoxin M1 in Parmesan cheese: HPLC determination. J. Food Sci., 59: 1313–1331. Go to original source...
    3. B ACIGALUPO M.A., I NS A., M ERONI G., D OVIS M., P ETRUZZELLI E. (1994): Determination of aflatoxin B1 in agricultural commodities by time-resolved fluoroimmuno-assay and immunoenzymatic assay. Analyst, 119: 2813–2815. Go to original source... Go to PubMed...
    4. BRADBURN N., COKER R.D., BLUNDEN G. (1995): A comparative study of solvent extraction efficiency and the performance of immunoaffinity and solid phase columns on the determination of aflatoxin B1. Food Chem., 52: 179–185. Go to original source...
    5. CVETNIC Z., PEPELJNJAK S. (1995): Aflatoxin-producing potential of Aspergillus flavus and Aspergillus parasiticus isolated from samples of smoked-dried meat. Nahrung, 39: 302–307. Go to original source... Go to PubMed...
    6. DESPHANDE S.S. (1994): Immunodiagnostics in agricultural, food and environmental quality control. Food Technol., 6: 136–139.
    7. DRAGACCI S., GLEIZES E., FREMY J.M., CANDLISH A.A.G. (1995): Use of immunoaffinity chromatography as a purification step for the determination of aflatoxin M1 in cheeses. Food Add. Contam., 12: 59–65. Go to original source... Go to PubMed...
    8. ESPINOSA E.T., ASKAR K.A., NACCCHA L.R., OLIVERA R.M., CASTRELLON J.P.S.A. (1996): Quantification of aflatoxins in corn distributed in the city of Monterrey, Mexico. Food Add. Contam., 12: 383–386. Go to original source... Go to PubMed...
    9. GOURAMA H., BULLERMAN L.B. (1995): Relationship between aflatoxin production and mold growth as measured by ergosterol and plate count. Lebensm.-Wiss. Technol., 28: 185–189. Go to original source...
    10. HAMILTON P.B. (1984): Determining safe levels of mycotoxin. J. Food Prot., 47: 570–573. Go to original source... Go to PubMed...
    11. JOANNON-KAKOURI E., CHRISTODOULIDOU M., CHRISTON E., CONSTANTINIDOU E. (1995): Immunoaffinity column/ HPLC determination of aflatoxin M1 in milk. Food Agric. Immunol., 7: 131–137. Go to original source...
    12. LEBIEDZIÑSKA A., GANOWIAK Z. (1995): Zawartoœae aflatoksyn w spo¿ywczych orzeszkach arachidowych. Przem. Spo¿., 3: 99–100.
    13. LESZCZYÑSKA J., MAS£OWSKA J., OWCZAREK A., PYTASZ U. (1997):, Determination of gluten content in non-gluten products. Chem. Anal., 42: 715–719.
    14. LESZCZYÑSKA J., MAS£OWSKA J., OWCZAREK A. (1998): Determination of pesticides in water and food by the ELISA method. Pol. J. Food Nutr. Sci., 7/48: 732–738.
    15. PEI-YIN WU, YUE-HORNG YEN, ROBIN Y.-Y. CHIOU (1995): Extraction of aflatoxin from peanut meal and kernels by CO2-methanol using a highly pressurized fluid extraction process. J. Food Prot., 58: 800–803. Go to original source... Go to PubMed...
    16. PESTKA J.J., ABOUZIED M.M., SUTKINO R. (1995): Immunological assay for mycotoxin detection. Food Technol., 2: 120–123.
    17. SABINO M., PURCHIO A., MILANEZ T.V. (1995): Aflatoxins B1, M1 and aflatoxicol in tissues and urine of calves receiving aflatoxin. Food Add. Contam., 12: 467–472. Go to original source... Go to PubMed...
    18. SCHATZKI T.F. (1995): Distribution of aflatoxin in pistachios. J. Agr. Food Chem., 43: 1561–1569. Go to original source...
    19. YAMASHITA A., YOSHIZAWA T., AIURA Y., SANCHEZ P.C., DIZON E.I., ARIM R.H. SARDJONO (1995): Fusarium mycotoxins and aflatoxins in corn from Southeast Asia. Biosci. Biotech. Biochem., 59: 1804–1807. Go to original source... Go to PubMed...
    20. YONG R.K. (1995): Nonspecific enzyme-linked immunosorbent assay for molds in foods. J. Food Sci., 60: 1357–1360.. Go to original source...

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