Vet Med - Czech, 2022, 67(8):395-407 | DOI: 10.17221/167/2020-VETMED

New perspectives in a microstructure study of the bovine and bubaline clawReview

BM Assis1, LPB Borges2, KF Pereira3, CR de Oliveira Lima4, LAF da Silva1, PPM Teixeira2, RE Rabelo3
1 Veterinary and Animal Science School, Goiás Federal University, Goiânia, GO, Brazil
2 Veterinary Institut, Pará Federal University, PA, Brazil
3 Veterinary Hospital, Goiás Federal University, Regional Jataí, Jataí, GO, Brazil
4 Veterinary Science School, Goiás State University of Jataí Campus, Jataí, GO, Brazil

The techniques of microtomography (Micro-CT), confocal laser scanner microscopy (CLSM), atomic force microscopy (AFM), nanoindentation - Vickers hardness (Nano-VH) and X-ray fluorescence (XRF) are undeniably important to the modern study of bovine podiatry. These techniques are also employed in engineering, physics and in the assessment of biomaterials used in reconstructive or experimental surgeries in bovine and bubaline claws. Although studies involving these analyses are still inconspicuous in veterinary medicine, these technologies represent a new paradigm in this area, enabling the development of new lines of research. The objective of this review is to gather information about the microstructural aspects of bovine and bubaline claws, concerning the intratubular and extratubular keratin, which is responsible for the physical and mechanical structure of the claw capsule. This study elucidates different methods used to evaluate the hooves of healthy and sick animals through a micrometric analysis and nano-scale analyses. We would like to emphasise that the described techniques can be applied to study other species.

Keywords: atomic force microscopy; confocal laser scanner microscopy; microtomography; nanoindentation; X-ray fluorescence

Received: August 24, 2020; Accepted: March 7, 2022; Prepublished online: May 27, 2022; Published: August 15, 2022  Show citation

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Assis B, Borges L, Pereira K, CR de Oliveira L, Silva LD, Teixeira P, Rabelo R. New perspectives in a microstructure study of the bovine and bubaline claw. Vet Med-Czech. 2022;67(8):395-407. doi: 10.17221/167/2020-VETMED.
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