Czech J. Anim. Sci., 2018, 63(4):144-151 | DOI: 10.17221/101/2017-CJAS

Effect of vitrification on apoptotic changes in feline embryosOriginal Paper

Małgorzata Ochota*, Wojciech Niżański
Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland

The aim of this study was to evaluate the influence of a vitrification-warming procedure on the viability of cat embryos and blastocysts and the incidence of apoptotic changes in blastocysts subjected to vitrification and blastocyst that developed from vitrified embryos. In the first part of the experiment, post-thaw embryo development and blastocyst viability were evaluated based on morphological appearance and the ability to develop (embryos) or re-expand (blastocyst) compared to control. In the second part, blastocysts that were viable after vitrification-warming and blastocysts that developed from vitrified-warmed embryos were stained with Annexin-V and TUNEL to evaluate apoptotic changes. Most of the vitrified-warmed embryos were viable after thawing, 36.3% developed to morula, and 14.7% to the blastocyst stage. The overall re-expansion rate of blastocysts that were vitrified on day 7 was 55.6%. Vitrification significantly increased apoptotic and necrotic changes in blastocysts, but did not influence late apoptotic and necrotic changes in blastocysts that developed from vitrified-warmed embryos. The total number of blastomeres in blastocysts was similar in blastocysts that developed from vitrified-warmed embryos (99.1 ± 23.1), but lower in blastocysts vitrified on day 7 (82.1 ± 16.8), if compared to the control group (107.9 ± 24.2). These results show that the vitrification-warming procedure returns embryos capable of further development to a good quality blastocyst in vitro. Whereas, vitrification of blastocysts caused the progression of apoptotic changes in some blastomeres, however it did not affect the overall blastocyst viability.

Keywords: cat; TUNEL; blastocyst; Annexin-V; apoptosis

Published: April 30, 2018  Show citation

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Ochota M, Niżański W. Effect of vitrification on apoptotic changes in feline embryos. Czech J. Anim. Sci.. 2018;63(4):144-151. doi: 10.17221/101/2017-CJAS.
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    References

    1. Ahn H.J., Sohn I.P., Kwon H.C., Jo D.H., Park Y.D., Min C.K. (2002): Characteristics of the cell membrane fluidity, actin fibers, and mitochondrial dysfunctions of frozen-thawed two-cell mouse embryos. Molecular Reproduction and Development, 61, 466-476. Go to original source... Go to PubMed...
    2. Anguita B., Vandaele L., Mateusen B., Maes D., Van Soom A. (2007): Developmental competence of bovine oocytes is not related to apoptosis incidence in oocytes, cumulus cells and blastocysts. Theriogenology, 67, 537-549. Go to original source... Go to PubMed...
    3. Betts D.H., King W.A. (2001): Genetic regulation of embryo death and senescence. Theriogenology, 1, 171-191. Go to original source... Go to PubMed...
    4. Brison D.R., Schultz R.M. (1998): Increased incidence of apoptosis in transforming growth factor α-deficient mouse blastocysts. Biology of Reproduction, 59, 136-144. Go to original source... Go to PubMed...
    5. Byrne A.T., Southgate J., Brison D.R., Leese H.J. (1999): Analysis of apoptosis in the preimplantation bovine embryo using TUNEL. Journal of Reproduction and Fertility, 117, 97-105. Go to original source... Go to PubMed...
    6. Chrenek P., Marakevich A.V., Kubovicova E. (2014): Developmental potential of vitrified rabbit embryos. Slovak Journal of Animal Science, 47, 198-201.
    7. Crichton E.G. (2002): Efficacy of porcine gonadotropins for repeated stimulation of ovarian activity for oocyte retrieval and in vitro embryo production and cryopreservation in Siberian tigers (Panthera tigris altaica). Biology of Reproduction, 68, 105-113. Go to original source... Go to PubMed...
    8. Dhali A., Anchamparuthy V., Butler S., Pearson R., Mullarky I., Gwazdauskas F. (2009): Effect of droplet vitrification on development competence, actin cytoskeletal integrity and gene expression in in vitro cultured mouse embryos. Theriogenology, 71, 1408-1416. Go to original source... Go to PubMed...
    9. Elnahas A., Alcolak E., Abu E., Elnahas T. (2010): Vitrification of human oocytes and different development stages of embryos: an overview. Middle East Fertility Society Journal, 15, 2-9. Go to original source...
    10. Fabian D., Gjorret J.O., Berthelot F., Martinat-Botte F., Maddox-Hyttel P. (2005a): Ultrastructure and cell death of in vivo derived and vitrified porcine blastocysts. Molecular Reproduction and Development, 70, 155-165. Go to original source... Go to PubMed...
    11. Fabian D., Koppel J., Maddox-Hyttel P. (2005b): Apoptotic processes during mammalian preimplantation development. Theriogenology, 64, 221-231. Go to original source... Go to PubMed...
    12. Fabian D., Makarevich A.V., Chrenek P., Bukovska A., Koppel J. (2007): Chronological appearance of spontaneous and induced apoptosis during preimplantation development of rabbit and mouse embryos. Theriogenology, 68, 1271-1281. Go to original source... Go to PubMed...
    13. Gjorret J.O., Knijn H.M., Dieleman S.J., Avery B., Larsson L.I., Maddox-Hyttel P. (2003): Chronology of apoptosis in bovine embryos produced in vivo and in vitro. Biology of Reproduction, 69, 1193-2000. Go to original source... Go to PubMed...
    14. Handyside A.H., Hunter S. (1986): Cell division and death in the mouse blastocyst before implantation. Wilhelm Roux's Archives of Developmental Biology, 195, 519-526. Go to original source... Go to PubMed...
    15. Hardy K. (1999): Apoptosis in the human embryo. Reviews of Reproduction, 4, 125-134. Go to original source... Go to PubMed...
    16. Kidson A., Rubio-Pomar F.J., Van Knegsel A., Van Tol H.T.A., Hazeleger W., Ducro-Steverink D.W., Colenbrander B., Dieleman S.J., Bevers M.M. (2004): Quality of porcine blastocysts produced in vitro in the presence or absence of GH. Reproduction, 127, 165-177. Go to original source... Go to PubMed...
    17. Makarevich A.V., Chrenek P., Massanyi P., Lukac N., Pivko J. (2008): Apoptosis detection as a tool for the determination of animal embryo quality. Slovak Journal of Animal Science, 41, 153-159.
    18. Mandawala A.A., Harvey S.C., Roy T.K., Fowler K.E. (2016): Cryopreservation of animal oocytes and embryos: current progress and future prospects. Theriogenology, 86, 1637-1644. Go to original source... Go to PubMed...
    19. Marquez-Alvarado Y.C., Galina C.S., Castilla B., Leon H., Moreno-Mendoza N. (2004): Evidence of damage in cryopreserved and fresh bovine embryos using the Tunel technique. Reproduction in Domestic Animals, 39, 141-145. Go to original source... Go to PubMed...
    20. Moussa M., Tremoleda J.L., Duchamp G., Bruyas J.F., Colenbrander B., Bevers M.M., Daels P.F. (2004): Evaluation of viability and apoptosis in horse embryos stored under different conditions at 5°C. Theriogenology, 61, 921-932. Go to original source... Go to PubMed...
    21. Nizanski W., Dejneka G.J., Klimowicz M., Dubiel A. (2005): Evaluating some properties of domestic cat epididymal spermatozoa and their cryopreservation. Medycyna Weterynaryjna, 61, 173-178. (in Polish)
    22. Ochota M., Pasieka A., Nizanski W. (2016a): Superoxide dismutase and taurine supplementation improves in vitro blastocyst yield from poor-quality feline oocytes. Theriogenology, 85, 922-927. Go to original source... Go to PubMed...
    23. Ochota M., Wojtasik B., Nizanski W. (2016b): Survival rate after vitrification of various stages of cat embryos and blastocyst with and without artificially collapsed blastocoels cavity. Reproduction in Domestic Animals, 52, 281-287. Go to original source... Go to PubMed...
    24. Pampfer S., Cordi S., Vanderheyden I., Van Der Smissen P., Courtoy P.J., Van Cauwenberge A., Alexandre H., Donnay I., De Hertogh R. (2001): Expression and role of Bcl-2 in rat blastocysts exposed to high d-glucose. Diabetes, 50, 143-149. Go to original source... Go to PubMed...
    25. Papis K. (2001): "Open" vitrification methods and their application in mammalian oocyte and embryo cryopreservation. Medycyna Weterynaryjna, 57, 547-551. (in Polish)
    26. Paschoal D.M., Sudano M.J., Schwarz K.R., Maziero R.R., Guastali M.D., Crocomo L.F., Magalhaes L.C., Martins Jr. A., Leal C.L., Landim-Alvarenga F.D. (2017): Cell apoptosis and lipid content of in vitro-produced, vitrified bovine embryos treated with forskolin. Theriogenology, 87, 108-114. Go to original source... Go to PubMed...
    27. Pederson M.J., Watson C.A., Blevins B.A., Loskutoff N.M. (2009): Domestic cat (Felis catus) embryo cryopreservation: slow-slowing versus vitrification. Reproduction Fertility and Development, 21, 180. Go to original source...
    28. Pope C.E., Gomez M.C., Galiguis J., Dresser B.L. (2012): Applying embryo cryopreservation technologies to the production of domestic and black-footed cats. Reproduction in Domestic Animals, 47, 125-129. Go to original source... Go to PubMed...
    29. Rizos D., Fair T., Papadopoulos S., Boland M.P., Lonergan P. (2002): Developmental, qualitative, and ultrastructural differences between ovine and bovine embryos produced in vivo or in vitro. Molecular Reproduction and Development, 62, 320-327. Go to original source... Go to PubMed...
    30. Saragusty J., Arav A. (2011): Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction, 141, 1-19. Go to original source... Go to PubMed...
    31. Tsujioka T., Otzdorff C., Braun J., Hochi S. (2008): Effect of post-IVF developmental kinetics on in vitro survival of vitrified-warmed domestic cat blastocysts. Theriogenology, 327, 323-327. Go to original source... Go to PubMed...
    32. Yang M.Y., Rajamahendran R. (2002): Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro. Animal Reproduction Science, 70, 159-169. Go to original source... Go to PubMed...

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