A lichen diversity survey of White Pine Hollow State Preserve in Dubuque County, Iowa, revealed the presence of 117 different lichens including 13 previously unrecorded for the state of Iowa, 72 previously unrecorded for Dubuque County, and one recorded in Iowa on only one prior occasion. This increases the number of lichens reported for the state of Iowa to a total of 478 and the number recorded for White Pine Hollow to 123. Lobaria pulmonaria (L.) Hoffm., an old-growth specialist that is facing declines around the globe, was not amongst the lichens observed although one of the last known collections (1901) of this lichen in Iowa occurred at White Pine Hollow. Efforts to propagate L. pulmonaria in a greenhouse setting as a means of providing insight to whether a reintroduction might be feasible were unsuccessful.