Pseudotrogulus funebris, new species, is described from Estação Biológica de Paranapiacaba (Santo André, State of São Paulo, Brazil). P. funebris differs from other species of the genus by the presence of a large number of tubercles on area I close to the median groove, area III with tubercles concentrated in the median region, tergite III with a large rhomboid tubercle and tarsus III-IV with 8 articles. Pseutrogulus is newly transferred to Hernandariinae based on the following characteristics: 1) median-anterior tubercles on anterior margin pointing upwards; 2) large tubercles on lateral-anterior margin; 3) eye mound with two tubercles upward.