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Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China

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Abstract

This study was conducted to determine genetic diversity and antimicrobial susceptibility profiles of Staphylococcus aureus recovered from bovine mastitis in Zhejiang Province, China. Out of 3178 quarter milk samples from 846 lactating cows, among which 459 cows (54.3%) were found HMT positive, 890 quarters (28%) were found having subclinical mastitis. From 75 representative S. aureus isolates, 16 distinct types were identified by pulsed-field gel electrophoresis (PFGE). Four major PFGE types (A, B, C, and D) accounted for 82.7% of all isolates, and type A (41.3%) was observed in multiple herds across the studied areas. Each region was found to have a predominant type: Hangzhou type A (64.1%), Ningbo type C (34.5%) and type B (23.1%), Jinhua type D (53.3%), and Taizhou type C (62.5%). Results of antimicrobial susceptibility tests showed that 90.7% of the isolates were resistant to at least one antimicrobial. Resistance to penicillin and ampicillin (77.3%), tetracycline (60.0%), or erythromycin (48.0%) was observed. The bacteria resistant to multiple antibiotics such as penicillin, ampicillin, tetracycline, and erythromycin were commonly found. The information obtained from this study is useful for designing specific control programs for bovine S. aureus mastitis in this region.

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Authors and Affiliations

  1. Department of Veterinary Medicine, College of Animal Science, Zhejiang University, Hangzhou, 310029, China

    Jian-ping Li, Lin Yuan, Ting He & Song-hua Hu

  2. National Institute for Communicable Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, 102206, China

    Hai-jian Zhou

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  1. Jian-ping Li
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  5. Song-hua Hu
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Correspondence to Song-hua Hu.

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Project (No. 2005C12015) supported by the Science and Technology Department of Zhejiang Province, China

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Li, Jp., Zhou, Hj., Yuan, L. et al. Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China. J. Zhejiang Univ. Sci. B 10, 753–760 (2009). https://doi.org/10.1631/jzus.B0920072

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  • DOI: https://doi.org/10.1631/jzus.B0920072

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