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Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells

虾青素对铜离子诱导的前列腺细胞氧化损伤的影响

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Abstract

Astaxanthin (AST), a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay; cell apoptosis and intracellular reactive oxygen species (ROS) levels were determined by flow cytometry; the mitochondrial membrane potential (MMP) was measured by fluorospectrophotometer; and activities of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were evaluated by a detection kit. The results show that copper ion (Cu2+) induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines (RWPE-1 and PC-3). AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu2+, but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu2+-induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells.

摘要

目的

研究虾青素对铜离子诱导的前列腺细胞氧化损伤 的影响,并探索其作用机制。

创新点

首次研究虾青素对铜离子诱导的前列腺细胞及前 列腺癌细胞氧化损伤的影响,并比较其对两种细 胞作用的差异。

方法

MTT 法测定铜离子与虾青素对前列腺细胞 (RWPE-1)和前列腺癌细胞(PC-3)生长的影 响;采用细胞流式仪测定虾青素对铜离子诱导的 RWPE-1 和PC-3 细胞凋亡的影响;荧光分光光度 法测定了虾青素对铜离子诱导的活性氧自由基 (ROS)产生的影响;采用罗丹明123(Rh123) 染色检测虾青素对铜离子诱导的细胞线粒体膜 电位(MMP)变化的影响;采用试剂盒测定了虾 青素对铜离子存在下丙二醛(MDA)含量、超氧 化物歧化酶(SOD)、过氧化氢酶(CAT)及谷 胱甘肽过氧化物酶(GSH-Px)活性变化的影响。

结论

结果表明,铜离子能诱导RWPE-1 和PC-3 细胞 凋亡,并伴随细胞内ROS 和MDA 含量升高;虾 青素处理可显著降低RWPE-1细胞中MDA含量, 升高线粒体膜电位,并保持ROS 含量稳定;虾青 素处理可降低PC-3 细胞中SOD、GSH-Px 和CAT 的活性,而对RWPE-1 细胞则作用相反。因此, 虾青素处理能有效降低铜离子对RWPE-1 细胞引 起的损伤,而通过降低抗氧化酶活性加剧铜离子 对PC-3 细胞的损伤。

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Correspondence to Sheng-rong Shen.

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Project supported by the Plan of Medicine and Health Science and Technology of the Zhejiang Province of China (No. 2012RCA022)

ORCID: Sheng-rong SHEN, http://orcid.org/0000-0003-4096-1693

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Meng, Hz., Ni, Xf., Yu, Hn. et al. Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells. J. Zhejiang Univ. Sci. B 18, 161–171 (2017). https://doi.org/10.1631/jzus.B1500296

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  • DOI: https://doi.org/10.1631/jzus.B1500296

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