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Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress

药用植物内生菌对作物生长及氧化应激的作用

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Abstract

Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the endophytic diversity of medicinal plants (Caralluma acutangula, Rhazya stricta, and Moringa peregrina) and the endophyte role in seed growth and oxidative stress. Various organs of medicinal plants yielded ten endophytes, which were identified as Phoma sp. (6 isolates), Alternaria sp. (2), Bipolaris sp. (1), and Cladosporium sp. (1) based on 18S rDNA sequencing and phylogenetic analysis. The culture filtrates (CFs; 25%, 50%, and 100% concentrations) from these endophytes were tested against the growth of normal and dwarf mutant rice lines. Endophytic CF exhibited dose-dependent growth stimulation and suppression effects. CF (100%) of Phoma sp. significantly increased rice seed germination and growth compared to controls and other endophytes. This growth-promoting effect was due to the presence of indole acetic acid in endophytic CF. The gas chromatography/mass spectrometry (GC/MS) analysis showed the highest indole acetic acid content ((54.31±0.21) μmol/L) in Bipolaris sp. In addition, the isolate of Bipolaris sp. exhibited significantly higher radical scavenging and anti-lipid peroxidation activity than the other isolates. Bipolaris sp. and Phoma sp. also exhibited significantly higher flavonoid and phenolic contents. The medicinal plants exhibited the presence of bio-prospective endophytic strains, which could be used for the improvement of crop growth and the mitigation of oxidative stresses.

摘要

目的

探讨药用植物内生菌的多样性及其在种子生长和氧化应激中的作用。

方法

从三种药用植物(Caralluma acutangula、Rhazya stricta 和 Moringa peregrina)中提取内生菌;基 于18S rDNA 测序和系统发育分析鉴定分离得到 的内生菌株;以正常和矮化突变体水稻品系为对 照,比较不同浓度的内生菌培养滤液(CF)对水 稻种子的发芽和生长的影响;通过气相色谱-质谱 分析CF 中的有效活性成分。

结论

从药用植物中共获得10 种内生菌,包括茎点霉 属6 株、链格孢属2 株、双极霉属1 株和枝孢霉 属1 株。CF 表现出剂量依赖性的生长刺激和抑制 作用。与对照和其他内生菌相比,100%的茎点霉 菌CF 显著促进了水稻种子的发芽和生长;双极 霉中的吲哚乙酸含量最高,并表现出比其更高的 自由基清除和抗脂质过氧化活性;双极霉菌和茎 点霉菌的类黄酮和酚类成分较高。综上所述,药 用植物中存在内生菌株,其可以用于改善作物生 长和减轻氧化应激。

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Authors and Affiliations

  1. UoN Chair of Oman’s Medicinal Plants & Marine Natural Products, University of Nizwa, Nizwa, 616, Oman

    Abdul Latif Khan, Khadija Al-Hosni, Salima Al-Khiziri, Liaqat Ali, Sang-Mo Kang & Ahmed Al-Harrasi

  2. Department of Biological Sciences & Chemistry, University of Nizwa, Nizwa, 616, Oman

    Syed Abdullah Gilani & Javid Hussain

  3. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea

    Muhammad Waqas, Sajjad Asaf, Raheem Shahzad & In-Jung Lee

  4. Department of Agriculture, Abdul Wali Khan University Mardan, Mardan, 23200, Pakistan

    Muhammad Waqas

  5. Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA

    Yoon-Ha Kim

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  1. Abdul Latif Khan
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  2. Syed Abdullah Gilani
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  3. Muhammad Waqas
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  4. Khadija Al-Hosni
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Correspondence to In-Jung Lee or Ahmed Al-Harrasi.

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Project supported by the Oman Research Council (FURAP Program), and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agriculture, Food and Rural Affairs Research Center Support Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (716001-7)

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ORCID: Ahmed AL-HARRASI, http://orcid.org/0000-0002-0815-5942

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Khan, A.L., Gilani, S.A., Waqas, M. et al. Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress. J. Zhejiang Univ. Sci. B 18, 125–137 (2017). https://doi.org/10.1631/jzus.B1500271

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