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Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum

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Abstract

Parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially expressed in the host pupae after 24-h parasitism. They were masquerade-like serine proteinase homolog (MSPH), enolase (Eno), bilin-binding protein (BBP), imaginal disc growth factor (IDGF), ornithine decarboxylase (ODC), cellular retinoic acid binding protein (CRABP), and one unknown function protein. The full length cDNA sequences of MSPH, Eno, and BBP were successfully cloned using rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the transcript levels of MSPH and BBP in the fat bodies of host pupae were inducible in response to the parasitism and their variations were consistent with translational changes of these genes after parasitism, while the transcript levels of Eno and IDGF were not affected by parasitism. This study will contribute to the better understanding of the molecular bases of parasitoidinduced host alterations associated with innate immune responses, detoxification, and energy metabolism.

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathology and Insects of Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310029, China

    Jia-ying Zhu, Qi Fang, Gong-yin Ye & Cui Hu

  2. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province and Key Laboratory of Southwest Mountain Forest Resources Conservation and Utilization of Ministry of Education, Southwest Forestry University, Kunming, 650224, China

    Jia-ying Zhu

Authors
  1. Jia-ying Zhu
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  2. Qi Fang
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  3. Gong-yin Ye
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  4. Cui Hu
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Correspondence to Gong-yin Ye.

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Project supported by the National Basic Research Program (973) of China (No. 2006CB102005), the National Natural Science Foundation of China (Nos. 30571251 and 30971959), the Zhejiang Provincial Natural Science Foundation of China (No. Z3090191), and the Program for New Century Excellent Talents in University of the Ministry of Education of China (No. NCET-05-0513)

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Zhu, Jy., Fang, Q., Ye, Gy. et al. Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum . J. Zhejiang Univ. Sci. B 12, 93–102 (2011). https://doi.org/10.1631/jzus.B1000158

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  • DOI: https://doi.org/10.1631/jzus.B1000158

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