Study of the monosaccharide composition of water-soluble polysaccharide complexes and pectic substances of Pimpinella anisum herbs

Authors

DOI:

https://doi.org/10.15587/2519-4852.2020.206776

Keywords:

biopolymers, WSPC, PS, Pimpinella anisum, laxative effect, drug "Senadex", herb, monosaccharides, quantitative determination, thin-layer chromatography, liquid chromatography

Abstract

In the study of the pharmacological activity of WSPC and pectin substances isolated from the Pimpinella anisum herbs, it was found that pectin substances are practically non-toxic and exhibit a pronounced laxative effect, not inferior to the comparison drug «Senadex».

The aim of the work. The study of the monosaccharide composition of water-soluble polysaccharide complexes and pectin substances isolated from Pimpinella anisum herbs.

Materials and methods. For analysis, we used Pimpinella anisum herbs, harvested in the summer of 2019 in Kharkov. The study was carried out by liquid chromatography on an Agilent 1290 liquid chromatograph, detection was refractometric.

Results and discussion. WSPC isolated from the Pimpinella anisum herbs contain two monosaccharides - glucose and ramnose. Rhamnose with a content of 215.5 mg / g is the dominant sugar, glucose is present in a much smaller amount - 17.5 mg / g. The glucose content in PS is approximately the same - 12.3 mg / g. Moreover, in pectins in the absence of ramnose, the presence of galactose and arabinose in the amount of 59.8 mg / g and 69.5 mg / g, respectively, was established.

Conclusions. Using liquid chromatography, the presence of two monosaccharides in WSPC and three monosaccharides in pectin isolated from Pimpinella anisum herbs was established

Author Biographies

Sergii Kolisnyk, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of Analytical Chemistry

Vadym Khanin, National Pharmaceutical University Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Associate Professor

Department of Industrial Pharmacy and Economics

Ulugbek Akbar ugli Umarov, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Postgraduate Student

Department of Analytical Chemistry

Oksana Koretnik, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Assistant

Department of Pharmaceutical Chemistry

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Published

2020-06-30

How to Cite

Kolisnyk, S., Khanin, V., Umarov, U. A. ugli, & Koretnik, O. (2020). Study of the monosaccharide composition of water-soluble polysaccharide complexes and pectic substances of Pimpinella anisum herbs. ScienceRise: Pharmaceutical Science, (3 (25), 33–38. https://doi.org/10.15587/2519-4852.2020.206776

Issue

No. 3 (25) (2020)

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Pharmaceutical Science

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Copyright (c) 2020 Sergii Kolisnyk, Vadym Khanin, Ulugbek Akbar ugli Umarov, Oksana Koretnik

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