The Effect of Coffee Fruit Skin Extract on Sperm Characteristics And Testicular of Mice With Ethanol-Induced


Haris Setiawan(1*), Rita Maliza(2), Syaiful Adam Maulana(3), Muhammad Ilham Hisbullah(4)

(1) Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830, Indonesia
(2) Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830,  
(3) Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830, Indonesia
(4) Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830, Indonesia
(*) Corresponding Author

Abstract


Coffee fruit skin contains antioxidant compounds that can repair damaged tissues, especially those of reproductive or-gans. This study was designed to assess to what extent the metha-nolic extract of coffee fruit skin affected sperm characteristics and testicular histology in mice receiving 15% ethanol for 15 days. It used 25 male mice aged four months and employed a completely randomized design with two controls (K) and three treatments (P), namely K1 (without ethanol), K2 (15% ethanol), P1 (15% ethanol and the methanolic extract of coffee fruit skin, or MECS, at 125 mg/ kg BW), P2 (15% ethanol and MECS 250 mg/kg BW), and P3 (15% ethanol and MECS 500 mg/kg BW). These treatments were administered orally with a 1 mL disposable syringe for 15 days. The parameters observed were sperm characteristics (viability, morphology, and sperm count) and the microscopic structure of the testicles (lu-men area, diameter, and area of seminiferous tubules). These data were then analyzed using the one-way ANOVA, continued with the LSD and Duncan’s tests (P <0.05). The results showed that P2 (250 mg/kg BW) substantially improved sperm count (86.5x105±1.73 cells/ mL), sperm viability (87.26±0.05%), and the proportion of normal sperm morphology (93.33±0.004%) of mice with 15% ethanol-in-duced sperm damages. Testicular histology also confirmed improve-ments in spermatogenic cells, as evident from the lumen, diameter, and area seminiferous tubules after receiving P1 and P2. In conclu-sion, the optimum dose of the coffee skin extract for improving sperm quality and microscopic structures of mice testicles is 250 mg/kg BW. 


Keywords


coffee skin extract, ethanol, reproductive system, sperm characteristics, testicular histology

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DOI: https://doi.org/10.15575/biodjati.v5i2.9280

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