Abstract
Human immune deficiency virus (HIV) continues to cause havoc around the globe and has a significant impact on public safety. Therefore, a convenient method for the rapid detection of HIV-DNA is the key to the treatment and prevention of this kind of disease. In this work, we constructed an ultrasensitive electrochemical biosensor for the rapid detection of HIV-DNA based on Nd–Sb-co-doped SnO2@Pt electrode material. The doping of Sb and Nd increased oxygen vacancies of the SnO2 crystal lattice, which greatly improved the conductivity of the material. Meanwhile, the spherical nanoflower structure of Nd–Sb-co-doped SnO2 not only provided abundant attachment sites for Pt nanoparticles (Pt NPs) but also avoided the aggregation of DNA strands. Hence, the biosensor exhibited high sensitivity, good stability, outstanding selectivity, wide detection ranges (from 1.0 × 10−13 to 1.0 × 10−7 M), as well as a low limit of detection (0.099 pM) for HIV-DNA.
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Acknowledgments
Authors thank Analysis and Measurement Center of Yunnan University for the sample testing service.
Funding
This work was supported by the National Natural Science Foundation of China (Grant Numbers 52061041, 51661033, 31660538, 51361028, and 51161025) and the 13th Graduate Student Scientific 336 Research Innovation Program of Yunnan University (Grant Number 2021Y384). This work was also supported by Workstation of Academician Chen Jing of Yunnan Province (No. 202105AF150012) and Free exploration fund for academician (No. 202205AA160007).
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EM contributed to writing of the original draft, experiment, investigation, and data curation. CL contributed to data management and investigation. XB contributed to investigation and data curation. PF contributed to investigation and data curation. GL contributed to investigation and data curation. KC contributed to investigation and data curation. LL contributed to supervision and resources. QQ contributed to conceptualization, supervision, and writing, reviewing, & editing of the manuscript.
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Ma, E., Liu, C., Bai, X. et al. An ultrasensitive electrochemical DNA biosensor based on the highly conductive Nd–Sb-co-doped SnO2@Pt nanocomposite for the rapid detection of HIV-DNA. Journal of Materials Research 37, 3617–3628 (2022). https://doi.org/10.1557/s43578-022-00731-x
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DOI: https://doi.org/10.1557/s43578-022-00731-x