Living neuronal network in neural systems communicate through synaptic connections. For aiming artificial control of the synaptic transmission in neuronal network, we have applied conventional optical tweezers using a focused near-infrared (NIR) laser. The optical trapping of neural cell adhesion molecules (NCAMs) labeled with quantum-dots (Q-dots) at the plasma membrane of living neuronal cells was achieved at high laser power (>300 mW) in our previous study; therefore, the further reduction of laser power was required. Here, we propose and demonstrate resonant optical tweezers of Q-dots attached to NCAM in a neuron by simultaneous irradiation with non-resonance and resonance laser beams. The optical trapping dynamics of them was revealed by fluorescence correlation spectroscopy (FCS). The transit times of Q-dots attached to NCAM at the focal spot increased with the simultaneous irradiation of NIR laser and resonance laser. These results suggest that the particle motion of Q-dots attached to NCAM was constrained at the focus spot due to optical trapping force enhanced with resonance laser irradiation. Our method is applicable to selective manipulation of signaling molecules in living neurons.
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