Abstract
Exposure to ultraviolet (UV) radiation is the main reason behind extrinsic skin aging. Changes due to chronic UV exposure are called photoaging. Natural products are effective ingredients against UV-mediated skin damage. Present study investigated the anti-photoaging properties of Camellia japonica flowers which possess various bioactivities. To enrich the extracts of C. japonica flowers, pectinase and beta-glucosidase treatment was employed. Anti-photoaging effect was screened using the changes in MMP-1 and collagen levels in UVA-irradiated human HaCaT keratinocytes. The crude extract of C. japonica flowers (CE) was shown to decrease the UVA-induced MMP-1 secretion while attenuating the collagen levels. Pectinase and beta-glucosidase treated CE (ECE) showed increased anti-photoaging effects against UVA-induced changes in MMP-1 and collagen production. Camellenodiol (CMD), a known triterpenoid from C. japonica, isolated as the active ingredient of ECE and its anti-photoaging effect was screened. Results showed that CMD ameliorated the UVA-induced deterioration in collagen levels by suppressing MMP-1 production in transcriptional level. CMD treatment downregulated the phosphorylation of p38, ERK, and JNK MAPKs along their downstream effectors, c-Fos, and c-Jun. In conclusion, enzyme-assisted extraction of C. japonica flowers was suggested to enhance the anti-photoaging properties suggestively through high bioactive content such as CMD.
Funding source: AMOREPACIFIC http://dx.doi.org/10.13039/100018547
Award Identifier / Grant number: SRT-01-R19E80012
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: This work was performed within the program of the AMOREPACIFIC Open Research (SRT-01-R19E80012) supported by a grant from AMOREPACIFIC.
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Conflict of interest statement: The authors declare that they have no competing interests.
References
1. D’Orazio, J, Jarrett, S, Amaro-Ortiz, A, Scott, T. UV Radiation and the skin. Int J Mol Sci 2013;14:12222–48. https://doi.org/10.3390/ijms140612222.Search in Google Scholar PubMed PubMed Central
2. Jin, HC, Jin, YS, Hai, RC, Lee, MK, Youn, CS, Rhie, G, et al.. Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol 2001;117:1218–24. https://doi.org/10.1046/j.0022-202x.2001.01544.x.Search in Google Scholar PubMed
3. Helfrich, YR, Sachs, DL, Voorhees, JJ. Overview of skin aging and photoaging. Dermatol Nurs 2008;20:177–83.Search in Google Scholar
4. Pittayapruek, P, Meephansan, J, Prapapan, O, Komine, M, Ohtsuki, M. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci 2016;17:868. https://doi.org/10.3390/ijms17060868.Search in Google Scholar PubMed PubMed Central
5. Freitas-Rodríguez, S, Folgueras, AR, López-Otín, C. The role of matrix metalloproteinases in aging: tissue remodeling and beyond. Biochim Biophys Acta Mol Cell Res 2017;1864:2015–25. https://doi.org/10.1016/j.bbamcr.2017.05.007.Search in Google Scholar PubMed
6. de Jager, TL, Cockrell, AE, Du Plessis, SS. Ultraviolet light induced generation of reactive oxygen species. Adv Exp Med Biol 2017;996:15–23. https://doi.org/10.1007/978-3-319-56017-5_2.Search in Google Scholar PubMed
7. Trautinger, F, Mazzucco, K, Knobler, RM, Trenz, A, Kokoschka, E-M. UVA- and UVB-induced changes in hairless mouse skin collagen. Arch Dermatol Res 1994;286:490–4. https://doi.org/10.1007/bf00371578.Search in Google Scholar PubMed
8. Yao, J, Liu, Y, Wang, X, Shen, Y, Yuan, S, Wan, Y, et al.. UVB radiation induces human lens epithelial cell migration via NADPH oxidase-mediated generation of reactive oxygen species and up-regulation of matrix metalloproteinases. Int J Mol Med 2009;24:153–9. https://doi.org/10.3892/ijmm_00000218.Search in Google Scholar PubMed
9. Wang, H, Kochevar, IE. Involvement of UVB-induced reactive oxygen species in TGF-β biosynthesis and activation in keratinocytes. Free Radic Biol Med 2005;38:890–7. https://doi.org/10.1016/j.freeradbiomed.2004.12.005.Search in Google Scholar PubMed
10. Fisher, GJ, Kang, S, Varani, J, Bata-Csorgo, Z, Wan, Y, Datta, S, et al.. Mechanisms of photoaging and chronological skin aging. Arch Dermatol 2002;138:1462–70. https://doi.org/10.1001/archderm.138.11.1462.Search in Google Scholar PubMed
11. Chen, DQ, Feng, YL, Cao, G, Zhao, Y-Y. Natural products as a source for antifibrosis therapy. Trends Pharmacol Sci 2018;39:937–52. https://doi.org/10.1016/j.tips.2018.09.002.Search in Google Scholar PubMed
12. Hu, L, Ying, J, Zhang, M, Qiu, X, Lu, Y. Antitumor potential of marine natural products: a mechanistic investigation. Anti Cancer Agents Med Chem 2018;18:702–18. https://doi.org/10.2174/1871520617666170918142811.Search in Google Scholar PubMed
13. Jiang, R-W, Lau, K-M, Hon, P-M, Mak, TC, Woo, KS, Fung, KP. Chemistry and biological activities of caffeic acid derivatives from Salvia miltiorrhiza. Curr Med Chem 2012;12:237–46. https://doi.org/10.2174/0929867053363397.Search in Google Scholar PubMed
14. Onodera, KI, Hanashiro, K, Yasumoto, T. Camellianoside, a novel antioxidant glycoside from the leaves of Camellia japonica. Biosci Biotechnol Biochem 2006;70:1995–8. https://doi.org/10.1271/bbb.60112.Search in Google Scholar PubMed
15. Piao, MJ, Yoo, ES, Koh, YS, Kang, HK., Kim, J, Kim, YJ, et al.. Antioxidant effects of the ethanol extract from flower of Camellia japonica via scavenging of reactive oxygen species and induction of antioxidant enzymes. Int J Mol Sci 2011;12:2618–30. https://doi.org/10.3390/ijms12042618.Search in Google Scholar PubMed PubMed Central
16. Kim, S, Jung, E, Shin, S, Kim, M-H, Kim, Y-S, Lee, J-S, et al.. Anti-inflammatory activity of Camellia japonica oil. BMB Rep 2012;45:177–82. https://doi.org/10.5483/bmbrep.2012.45.3.177.Search in Google Scholar PubMed
17. Lee, JH, Kim, JW, Ko, NY, Mun, S-H, Kim, D-K, Kim, J-D, et al.. Camellia japonica suppresses immunoglobulin E-mediated allergic response by the inhibition of SYK kinase activation in mast cells. Clin Exp Allergy 2008;38:794–804. https://doi.org/10.1111/j.1365-2222.2008.02936.x.Search in Google Scholar PubMed
18. Yoshikawa, M, Morikawa, T, Fujiwara, E, Ohgushi, T, Asao, Y, Matsuda, H. New noroleanane-type triterpene saponins with gastroprotective effect and platelet aggregation activity from the flowers of Camellia japonica: revised structures of camellenodiol and camelledionol. Heterocycles 2001;55:1653–7. https://doi.org/10.3987/com-01-9284.Search in Google Scholar
19. Oh, JH, Seo, Y, Kong, C. Anti‐photoaging effects of solvent‐partitioned fractions from Portulaca oleracea L. on UVB‐stressed human keratinocytes. J Food Biochem 2019;43:e12814. https://doi.org/10.1111/jfbc.12814.Search in Google Scholar PubMed
20. Lee, JJ, Kim, KB, Heo, J, Cho, D-H, Kim, H-S, Han, SH, et al.. Protective effect of Arthrospira platensis extracts against ultraviolet B-induced cellular senescence through inhibition of DNA damage and matrix metalloproteinase-1 expression in human dermal fibroblasts. J Photochem Photobiol B Biol 2017;173:196–203. https://doi.org/10.1016/j.jphotobiol.2017.05.042.Search in Google Scholar PubMed
21. Puri, M, Sharma, D, Barrow, CJ. Enzyme-assisted extraction of bioactives from plants. Trends Biotechnol 2012;30:37–44. https://doi.org/10.1016/j.tibtech.2011.06.014.Search in Google Scholar PubMed
22. Azmir, J, Zaidul, ISM, Rahman, MM, Sharif, KM, Mohamed, A, Sahena, F, et al.. Techniques for extraction of bioactive compounds from plant materials: a review. J Food Eng 2013;117:426–36. https://doi.org/10.1016/j.jfoodeng.2013.01.014.Search in Google Scholar
23. Anand, M, Van Meter, TE, Fillmore, HL. Epidermal growth factor induces matrix metalloproteinase-1 (MMP-1) expression and invasion in glioma cell lines via the MAPK pathway. J Neuro Oncol 2011;104:679–87. https://doi.org/10.1007/s11060-011-0549-x.Search in Google Scholar PubMed
24. Hwang, YP, Oh, KN, Yun, HJ, Jeong, HG. The flavonoids apigenin and luteolin suppress ultraviolet A-induced matrix metalloproteinase-1 expression via MAPKs and AP-1-dependent signaling in HaCaT cells. J Dermatol Sci 2011;61:23–31. https://doi.org/10.1016/j.jdermsci.2010.10.016.Search in Google Scholar PubMed
25. Numazawa, S, Watabe, M, Nishimura, S, Kurosawa, M, Izuno, M, Yoshida, T. Regulation of ERK-mediated signal transduction by p38 MAP kinase in human monocytic THP-1 cells. J Biochem 2003;133:599–605. https://doi.org/10.1093/jb/mvg077.Search in Google Scholar PubMed
26. Plotnikov, A, Zehorai, E, Procaccia, S, Seger, R. The MAPK cascades: signaling components, nuclear roles and mechanisms of nuclear translocation. Biochim Biophys Acta Mol Cell Res 2011;1813:1619–33. https://doi.org/10.1016/j.bbamcr.2010.12.012.Search in Google Scholar PubMed
27. Xu, QF, Zheng, Y, Chen, J, Xu, X-Y, Gong, Z-J, Huang, Y-F, et al.. Ultraviolet A enhances cathepsin L expression and activity via JNK pathway in human dermal fibroblasts. Chin Med J 2016;129:2853–60. https://doi.org/10.4103/0366-6999.194654.Search in Google Scholar PubMed PubMed Central
28. Karin, M. The regulation of AP-1 activity by mitogen-activated protein kinases. J Biol Chem 1995;270:16483–6. https://doi.org/10.1074/jbc.270.28.16483.Search in Google Scholar PubMed
29. Thao, NTP, Hung, TM, Cuong, TD, Kim, JC, Kim, EH, Jin, SE, et al.. 28-Nor-oleanane-type triterpene saponins from Camellia japonica and their inhibitory activity on LPS-induced NO production in macrophage RAW264.7 cells. Bioorg Med Chem Lett 2010;20:7435–9. https://doi.org/10.1016/j.bmcl.2010.10.013.Search in Google Scholar PubMed
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