Abstract
The aim of this study was to investigate the effects of exogenously added pancreatic phos-pholipase A2 (pPLA2) on the production of reactive oxygen species by human polymorpho nuclear leukocytes (PMNs). Pancreatic PLA2 was used because PMNs do not possess a re ceptor for that enzyme and, therefore, the receptor-mediated effects could be excluded. Respiratory burst activity of PMNs was monitored by luminol-amplified chemiluminescence and the lipid composition of neutrophils after treatment with pPLA2 was determined by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Our results show that the products of the pPLA2 digestion of the PMN membrane -lysophospholipids and the corresponding free fatty acids -significantly enhanced the respiratory burst response of human neutrophils.
© 1946 – 2014: Verlag der Zeitschrift für Naturforschung
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
