O2 evolution activity of PS II in chloroplast stored in buffer including various supercooling solvents was studied. Viscous solvents such as polyethylene glycol (PEG) was remarkably effective for the increase of lifetime of intact chloroplast. Lifetime of immobilized chloroplast by means of radiation polymerization was prolonged more than 40 days in buffer including PEG 70% (v/v). It was found that viscous solvents having PEG units in molecular chain were effective and methoxypolyethyleneglycol methacrylate monomer (M-23G) was most suitable as a immobilization carrier. The immobilized chloroplast with M-23G retained the high activity yield more than 30 days in buffer including PEG.
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