When reovirus-infected Hela cells are incubated at 43 °C virus-specific messenger RNA is released from the polysomes. It accumulates free in the cytoplasm as messenger ribonucleopro- tein particles (mRNPs). These particles have a sedimentation rate of about 50 S and a buoyant density in CsCl of 1.42 g/cm3. Reovirus mRNPs contain, besides all three size classes of reovirus messenger RNA. the same spectrum of proteins found in the polysomal mRNPs from uninfected cells, plus two additional proteins with molecular masses of 70000 d and 110000 d, respectively. Electron microscopic examination of the reovirus mRNP fraction reveals specific Y-shaped structures with a total mean length of 0.5 μm.
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