Hydrodynamic shearing forces appearing during nucleoprotein gel-filtration on Sephadex G-25 cause a disruption of the macromolecular structure in nucleoproleins.
The effect of these shearing forces can be observed both in electron micrographs and by electrophoretic analysis.
The decrease of the molecular weight and also the changes in the electronegative charge,molecular weight ratio cause an increase of the electrophoretic mobility of the gel-filtered highly polymerized DNA and of the RNA complexed to it compared with the not gel-filtered nucleoprotein.
Received: 1969-10-5
Published Online: 2014-6-2
Published in Print: 1970-4-1
© 1946 – 2014: Verlag der Zeitschrift für Naturforschung
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