Abstract
Objectives
This study aims to evaluate the antioxidant and antiviral potency of n-hexane, ethyl acetate and, water fractions of Begonia medicinalis Ardi & D.C.Thomas as well as to identify the chemical constituents.
Methods
Assays for antioxidant and antiviral activity (HIV-1) were carried out on MT-4 cells infected with HIV using the DPPH method and the determination of the cytopathic effect. Meanwhile, GC-MS was used to identify the chemical compounds.
Results
The determination of antioxidants showed that all fractions possessed potent activity with the IC50 ranging from 2.61 to 8.26 μg/mL. From the antiviral activity of MT-4 cells infected by HIV, the n-hexane fraction of B. medicinalis showed the most potency with the IC50 of 0.04 ± 0.05 μg/mL. It has less cytotoxicity (11.08 ± 4.60 μg/mL) affording the high selectivity index of 238.80. Furthermore, GC-MS analysis of n-hexane fraction found the major compound of carboxylic acid derivate with the area percentage of 76.4% and the presence of phenolic compounds (8.38%). Meanwhile, in water fraction, terpenoids were found in a higher concentration (10.05%) than others.
Conclusions
Therefore, this study supports the application of B. medicinalis as a herbal medicine for antioxidant and antiviral.
Funding source: General Directorate of Research and Development
Funding source: Ministry of Research and Technology
Acknowledgments
The authors would like to greatly acknowledge the General Directorate of Research and Development, the Ministry of Research and Technology, Republic of Indonesia, for supporting this study through Applied Research grant with the Contract Number 308/UN28.2/PL/2020. The Authors also acknowledge Ms. Melia Kurniawati who help in this study.
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Research funding: Applied research grant from General Directorate of Research and Development, the Ministry of Research and Technology, Republic of Indonesia.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: Not applicable.
References
1. UNAIDS: mission and roles. Glob AIDSnews 1995;2–3.Search in Google Scholar
2. UNAIDS. Global HIV & AIDS statistics – 2020 fact sheet. Available from: https://www.unaids.org/en/resources/fact-sheet [Accessed 25 Sept 2020].Search in Google Scholar
3. Edziri, H, Mastouri, M, Aouni, M, Verschaeve, L. Polyphenols content, antioxidant and antiviral activities of leaf extracts of Marrubium deserti growing in Tunisia. South Afr J Bot 2012;80:104–9. https://doi.org/10.1016/j.sajb.2012.03.001.Search in Google Scholar
4. Lin, CC, Cheng, HY, Yang, CM, Lin, TC. Antioxidant and antiviral activities of Euphorbia thymifolia L. J Biomed Sci 2002;9:656–64. https://doi.org/10.1159/000067281.Search in Google Scholar
5. Sokmen, M, Serkedjieva, J, Daferera, D, Gulluce, M, Polissiou, M, Tepe, B, et al.. In vitro antioxidant, antimicrobial and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. J Agric Food Chem 2004;52:3309–12. https://doi.org/10.1021/jf049859g.Search in Google Scholar
6. Ardi, WH, Zubair, MS, Ramadanil, Thomas, DC. Begonia medicinalis (Begoniaceae), a new species from Sulawesi, Indonesia. Phytotaxa 2019;423:41–5. https://doi.org/10.11646/phytotaxa.423.1.5.Search in Google Scholar
7. Anam, S, Yuliet, RA, Dwimurti, F, Rismayanti, D, Zubair, MS. Cytotoxic activity of methanol extract of Benalu Batu (Begonia sp.): an ethnomedicine of Wana Tribe-Central Sulawesi. Indo J Pharm Sci 2014;2:10–16.Search in Google Scholar
8. Zubair, MS, Alarif, WM, Ghandourah, MA, Anam, S, Jantan, I. Cytotoxic activity of 2-O-β-glucopyranosilcucurbitacin D from Benalu Batu (Begonia sp.) growing in Morowali, Central Sulawesi. Indo J Chem 2020;20:766–72. https://doi.org/10.22146/ijc.43626.Search in Google Scholar
9. Zubair, MS, Anam, S, Yuliet, KA, Hidayat, M, Ridhay, A. Molecular docking approach to identify potential anticancer compound from Benalu batu (Begonia sp.) AIP Conf Proc 2016;1755:080005-1–080005-7.10.1063/1.4958513Search in Google Scholar
10. Zubair, MS, Alarif, WM, Ghandourah, MA, Anam, S. A new steroid glycoside from Begonia sp.: cytotoxic activity and docking studies. Nat Prod Res 2019. https://doi.org/10.1080/14786419.2019.1669026.Search in Google Scholar
11. Sulastri, E, Zubair, MS, Anas, NI, Abidin, S, Hardani, R, Yulianti, R, et al.. Total phenolic, total flavonoid, Quercetin content and antioxidant activity of Standardized extract of Moringa oleifera leaf from regions with different elevation. Phcog J 2018;10(6 Suppl):s104–8. https://doi.org/10.5530/pj.2018.6s.20.Search in Google Scholar
12. Smee, DF, Hurst, BL, Evans, WJ, Clyde, N, Wright, S, Peterson, C, et al.. Evaluation of cell viability dyes in antiviral assays with RNA viruses that exhibit different cytopathogenic properties. J Virol Methods 2017;246:24651–7. https://doi.org/10.1016/j.jviromet.2017.03.012.Search in Google Scholar
13. Widodo, A, Widiyanti, P, Prajogo, B. Antiviral activity of Justicia gendarussa Burm.f. leaves against HIV-infected MT-4 cells. Afr J Infect Dis 2018;12:36–43. https://doi.org/10.21010/ajid.v12i1s.4.Search in Google Scholar
14. Cotelle, N, Bernier, JL, Catteau, JP, Pommery, J, Wallet, JC, Gaydou, EM. Antioxidant properties of hydroxy-flavones. Free Radic Biol Med 1996;20:35–43. https://doi.org/10.1016/0891-5849(95)02014-4.Search in Google Scholar
15. Maddry, JA, Chen, X, Jonsson, CB, Ananthan, S, Hobrath, J, Smee, DF, et al.. Discovery of novel benzoquinazolinones and thiazoloimidazoles, inhibitors of influenza H5N1 and H1N1 viruses, from a cell-based high-throughput screen. J Biomol Screen 2011;16:73–81. https://doi.org/10.1177/1087057110384613.Search in Google Scholar
16. Hwang, SW, Ha, TJ, Lee, JR, Lee, J, Nam, SH, Park, KH, et al.. Isolation of anthraquinone derivatives from the root of Rumex japonicus H. J Korean Soc Appl Biol Chem 2004;47:274–8.Search in Google Scholar
17. Khumaidi, A, Widodo, A, Nugrahani, AW, Sasmito, E, Fakhrudin, N. Lymphocyte cell proliferation profile of Begonia medicinalis, from North Morowali Regency Central Sulawesi Province. Indo J Pharm Sci 2020;18:61–7.Search in Google Scholar
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