Abstract
Background
Polyphenols are known because of their phytochemical constituents having antioxidative properties. In this regard, grape juice is highly enriched with polyphenolic constituents, and its supplementation has been known to improve many health and age-associated diseases and risk factors. Our study was entirely dedicated to evaluating the positive effects of grape juice on young and old rats’ erythrocytes and plasma.
Methods
Young (4 months) and old (24 months) male Wistar rats were given an oral dose of grape juice for 28 days. They were grouped into four categories (n = 6): Group I: young control rats; Group II: young grape juice treated rats; Group III: old control rats; Group IV: old treated rats. The treated groups were administered with 10 μL/g of grape juice according to body weight. The following biomarkers of antioxidant defense were measured: ferric reducing ability of plasma (FRAP), reactive oxygen species (ROS), plasma membrane redox system (PMRS), glutathione (GSH), osmotic fragility, and the decrease in lipid peroxidation measured in terms of malondialdehyde (MDA) levels.
Results
A significant increase (p<0.05) in antioxidant levels of FRAP, PMRS, and GSH and a significant decrease (p<0.05) in oxidized products such as ROS and MDA were seen in the treated rats in comparison to the controls. The decrease in ROS and rise in FRAP and PMRS levels suggest the ability of grape juice to combat oxidative stress effectively.
Conclusion
We propose the role of grape juice as a potent antioxidant because of its easy bioavailability and its role in combating stress. Our results also approve grape juice as a possible antiaging agent.
Funding source: Science and Engineering Research Board
Award Identifier / Grant number: EMR/2016/006470
Funding statement: This work was supported by a research grant to SIR from SERB-DST, Govt of India (EMR/2016/006470, Funder Id: http://dx.doi.org/10.13039/501100001843). The work has further been supported by a research grant to Sukanya Bhoumik from Junior Research Fellowship, Council of Scientific and Industrial Research.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: The authors declare no conflict of interest.
Ethical approval: Research involving animals complied with all relevant national regulations and institutional policies (IAEC/AU/2019(1)/09) for the care and use of animals. (839/GO/Re/04/CPCSEA).
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