Abstract
Background
Obesity has been implicated in impaired salivary secretion. This study aimed at evaluating the influence of diet-induced obesity on salivary secretion and how re-feeding with normal diet would affect changes in salivary secretion associated with diet-induced obesity.
Methods
Weaning rats weighing 55–65 g were randomly divided into three groups (control, diet-induced obese, re-fed obese) of seven rats each. The diet-induced obese group was fed a high-fat diet for 15 weeks, whereas the re-fed obese group received normal diet for another 15 weeks following the 15 weeks of high-fat diet. After treatment, blood and stimulated saliva samples were collected for the analyses of total protein, electrolytes, amylase, Immunoglobulin A (IgA), leptin and ghrelin. Tissue total protein, nitric oxide level, expressions of Na+/K+-ATPase, muscarinic (M3) receptor and aquaporin 5 in the submandibular glands were determined. Data were presented as mean±SEM and compared using independent student t-test and ANOVA with Tukey’s post-hoc test.
Results
Results indicated increases in the levels of salivary calcium, phosphate, bicarbonate and leptin, whereas the levels of salivary amylase and ghrelin showed reduction in the obese group compared with the control. Most of these changes were reversed in the re-fed obese group. There were no significant differences in salivary lag time, flow rate, levels of tissue total protein, nitric oxide and the relative expressions of M3 receptor, Na++/K+-ATPase and aquaporin 5 in the submandibular glands between the obese and control groups.
Conclusions
Diet-induced obesity lead to some changes in salivary factors which were reversed by returning to normal diet.
Acknowledgment
This manuscript is part of the PhD work of T.J. Lasisi in the Department of Physiology, University of Ibadan.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved its submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Zolotukhin S. Metabolic hormones in saliva: origins and functions. Oral Dis 2013;19:219–29.10.1111/odi.12015Search in Google Scholar PubMed PubMed Central
2. Bohlender J, Rauh M, Zenk J, Gröschl M. Differential distribution and expression of leptin and the functional leptin receptor in major salivary glands of humans. J Endocrinol 2003;178:217–23.10.1677/joe.0.1780217Search in Google Scholar PubMed
3. Ohta K, Laborde NJ, Kajiya M, Shin J, Zhu T, Thondukolam AK, et al. Expression and possible immune-regulatory function of ghrelin in oral epithelium. J Dent Res 2011;90:1286–92.10.1177/0022034511420431Search in Google Scholar PubMed PubMed Central
4. Satish BN, Srikala P, Maharudrappa B, Awanti SM, Kumar P, Hugar D. Saliva: a tool in assessing glucose levels in Diabetes Mellitus. J Int Oral Health 2014;6:114–7.Search in Google Scholar PubMed
5. Lasisi TJ, Raji YR, Salako BL. Salivary creatinine and urea analysis in patients with chronic kidney disease: A case control study. BMC Nephrol 2016;17:1–6.10.1186/s12882-016-0222-xSearch in Google Scholar PubMed PubMed Central
6. Guo L, Shi W. Salivary biomarkers for caries risk assessment. J Calif Dent Assoc 2013;41:107–9, 112–8.10.1080/19424396.2013.12222284Search in Google Scholar PubMed
7. Ebersole JL, Nagarajan R, Akers D, Miller CS. Targeted salivary biomarkers for discrimination of periodontal health and disease(s). Front Cell Infect Microbiol 2015;5:62.10.3389/fcimb.2015.00062Search in Google Scholar PubMed PubMed Central
8. Gualtero DF, Suarez Castillo A. Biomarkers in saliva for the detection of oral squamous cell carcinoma and their potential use for early diagnosis: a systematic review. Acta Odontol Scand 2016;74:170–7.10.3109/00016357.2015.1110249Search in Google Scholar PubMed
9. Marosti AR, de Almeida FN, de Moraes SM, Molinari SL, Natali MR. Effects of the cafeteria diet on the salivary glands of trained and sedentary Wistar rats. Acta Scientiarum. Biological Sciences 2012;34:113–8.10.4025/actascibiolsci.v34i1.7473Search in Google Scholar
10. Rodrigues L, Mouta R, Costa AR, Pereira A, Capela e Silva F, Amado F, et al. Effects of high-fat diet on salivary α-amylase, serum parameters and food consumption in rats. Arch Oral Biol 2015;60:854–62.10.1016/j.archoralbio.2015.02.015Search in Google Scholar PubMed
11. Ribeiro DL, Pinto ME, Maeda SY, Taboga SR, Góes RM. High fat-induced obesity associated with insulin-resistance increases FGF-2 content and causes stromal hyperplasia in rat ventral prostate. Cell Tissue Res 2012;349:577–88.10.1007/s00441-012-1420-xSearch in Google Scholar PubMed
12. Novelli EL, Diniz YS, Galhardi CM, Ebaid GM, Rodrigues HG, Mani F, et al. Anthropometrical parameters and markers of obesity in rats. Lab Anim 2007;41:111–9.10.1258/002367707779399518Search in Google Scholar PubMed
13. Mutiso SK, Rono DK, Bukachi F. Relationship between anthropometric measures and early electrocardiographic changes in obese rats. BMC Research Notes 2014;7:931–7.10.1186/1756-0500-7-931Search in Google Scholar PubMed PubMed Central
14. Choi JS, Park IS, Kim SK, Lim JY, Kim YM. Analysis of age related changes in the functional morphologies of salivary glands in mice. Arch Oral Biol 2013;58:1635–42.10.1016/j.archoralbio.2013.07.008Search in Google Scholar PubMed
15. Lasisi TJ, Shittu ST, Oguntokun MM, Tiamiyu NA. Aging affects morphology but not stimulated secretion of saliva in rats. Ann Ib Postgrad Med 2014;12:109–14.Search in Google Scholar PubMed
16. Romero AC, Bergamaschi CT, de Souza DN, Nogueira FN. Salivary alterations in rats with experimental chronic kidney disease. PLoS One 2016;11:e0148742.10.1371/journal.pone.0148742Search in Google Scholar PubMed
17. Trinder P. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol 1969;22:158–61.10.1136/jcp.22.2.158Search in Google Scholar PubMed
18. Gordon T, Castelli WP, Hjortland MC, Kannel WB, Dawber TR. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am J Med 1977;62: 707–14.10.1016/0002-9343(77)90874-9Search in Google Scholar PubMed
19. Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem 1949;177:751–66.10.1016/S0021-9258(18)57021-6Search in Google Scholar PubMed
20. Trinder P. A rapid method for the determination of sodium in serum. Analyst 1951;76:596–99.10.1039/an9517600596Search in Google Scholar
21. Kessler G, Wolfman M. An automated procedure for the simultaneous determination of calcium and phosphorus. Clin Chem 1964;10:686–703.10.1093/clinchem/10.8.686Search in Google Scholar PubMed
22. Schoenfeld RG, Lewellen CJ. Acolorimetric method for determination of serum chloride. Clin Chem 1964;10:533–9.10.1093/clinchem/10.6.533Search in Google Scholar
23. Tietz NW, editor. Fundamentals of clinical chemistry. Philadelphia: W. B. Saunders Co., 1982:865.Search in Google Scholar
24. Schrauwen P, Westerterp KR. The role of high-fat diets and physical activity in the regulation of body weight. Br J Nutr 2000;84:417–27.10.1017/S0007114500001720Search in Google Scholar PubMed
25. Kurek K, Mikłosz A, Łukaszuk B, Chabowski A, Górski J, Żendzian-Piotrowska M. Inhibition of Ceramide De Novo Synthesis Ameliorates Diet Induced Skeletal Muscles Insulin Resistance. J Diabetes Res 2015;2015:154762.10.1155/2015/154762Search in Google Scholar PubMed PubMed Central
26. Matczuk J, Zalewska A, Łukaszuk B, Knaś M, Maciejczyk M, Garbowska M, et al. Insulin resistance and obesity affect lipid profile in the salivary glands. J Diabetes Res 2016;2016:8163474.10.1155/2016/8163474Search in Google Scholar PubMed PubMed Central
27. Schwab U, Lauritzen L, Tholstrup T, Haldorssoni T, Riserus U, Uusitupa M, et al. Effect of the amount and type of dietary fat on risk factors for cardiovascular diseases, and risk of developing type 2 diabetes, cardiovascular diseases, and cancer: a systematic review. Food Nutr Res 2014;58.10.3402/fnr.v58.25145Search in Google Scholar PubMed PubMed Central
28. de Souza RJ, Mente A, Maroleanu A, Cozma AI, Ha V, Kishibe T, et al. Intake of saturated and trans unsaturated fatty acids and risk of all-cause mortality, cardiovascular disease, and type 2 diabetes: systematic review and meta-analysis of observational studies. Br Med J 2015;351:h3978.10.1136/bmj.h3978Search in Google Scholar PubMed PubMed Central
29. Ittichaicharoen J, Apaijai N, Tanajak P, Sa-Nguanmoo P, Chattipakorn N, Chattipakorn SC. Impaired mitochondria and intracellular calcium transients in the salivary glands of obese rats. Appl Physiol Nutr Metab 2017;42:420–29.10.1139/apnm-2016-0545Search in Google Scholar PubMed
30. Hartman M-L, Groppo F, Ohnishi M, Goodson JM, Hasturk H, Tavares M. Can salivary phosphate levels be an early biomarker to monitor the evolvement of obesity? Cont Nephrol 2013;180:138–48.10.1159/000346793Search in Google Scholar PubMed PubMed Central
31. Hazen SP. Supragingival dental calculus. Periodontol 2000 1995;8:125–36.10.1111/j.1600-0757.1995.tb00050.xSearch in Google Scholar PubMed
32. Jin Y, Yip HK. Supragingival calculus: formation and control. Crit Rev Oral Biol Med 2002;13:426–41.10.1177/154411130201300506Search in Google Scholar PubMed
33. de Castilhos ED, Horta BL, Gigante DP, Demarco FF, Peres KG, Peres MA. Association between obesity and periodontal disease in young adults: a population-based birth cohort. J Clin Periodontol 2012;39:717–24.10.1111/j.1600-051X.2012.01906.xSearch in Google Scholar PubMed PubMed Central
34. Susin C, Haas AN, Valle PM, Oppermann RV, Albandar JM. Prevalence and risk indicators for chronic periodontitis in adolescents and young adults in south Brazil. J Clin Periodontol 2011;38:326–33.10.1111/j.1600-051X.2011.01699.xSearch in Google Scholar PubMed
35. Falchi M, El-Sayed Moustafa JS, Takousis P, Pesce F, Bonnefond A, Andersson-Assarsson JC, et al. Low copy number of the salivary amylase gene predisposes to obesity. Nat Genet 2014;46:492–7.10.1038/ng.2939Search in Google Scholar PubMed PubMed Central
36. Aguirre M, Jonkers DM, Troost FJ, Roeselers G, Venema K. In vitro characterization of the impact of different substrates on metabolite production, energy extraction and composition of gut microbiota from lean and obese subjects. PLoS One 2014;9:e113864.10.1371/journal.pone.0113864Search in Google Scholar PubMed
37. Scarpace PJ, Zhang Y. Leptin resistance: a prediposing factor for diet-induced obesity. Am J Physiol Regul Integr Comp Physiol 2009;296:R493–500.10.1152/ajpregu.90669.2008Search in Google Scholar PubMed
38. Hill JO, Dorton J, Sykes MN, Digirolamo M. Reversal of dietary obesity is influenced by its duration and severity. Int J Obes 1989;13:711–22.Search in Google Scholar PubMed
39. Wang HT, Liu CF, Tsai TH, Chen YL, Chang HW, Tsai CY, et al. Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice. J Transl Med 2012;10:145.10.1186/1479-5876-10-145Search in Google Scholar PubMed
40. Guo J, Jou W, Gavrilova O, Hall KD. Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets. PLoS One 2009;4:e5370.10.1371/journal.pone.0005370Search in Google Scholar PubMed
41. Briggs DI, Lockie SH, Wu Q, Lemus MB, Stark R, Andrews ZB. Calorie-restricted weight loss reverses high-fat diet-induced ghrelin resistance, which contributes to rebound weight gain in a ghrelin-dependent manner. Endocrinol 2013;154:709–17.10.1210/en.2012-1421Search in Google Scholar
42. Littlejohns B, Lin H, Angelini GD, Halestrap AP, Suleiman MS. Switching back to normal diet following high-fat diet feeding reduces cardiac vulnerability to ischaemia and reperfusion injury. Cell Physiol Biochem 2014;34:1090–100.10.1159/000366323Search in Google Scholar PubMed
43. Bourdiol P, Mioche L, Monier S. Effect of age on salivary flow obtained under feeding and non-feeding conditions. J Oral Rehabil 2004;31:445–52.10.1111/j.1365-2842.2004.01253.xSearch in Google Scholar PubMed
44. Shern RJ, Fox PC, Li SH. Influence of age on the secretory rates of the human minor salivary glands and whole saliva. Arch Oral Biol 1993;38:755–61.10.1016/0003-9969(93)90071-SSearch in Google Scholar PubMed
45. Filippi BM, Lam KT. Leptin and aging. Aging (Albany NY) 2014;6:82–3.10.18632/aging.100637Search in Google Scholar PubMed PubMed Central
46. Balaskó M, Soós S, Székely M, Pétervári E. Leptin and aging: review and questions with particular emphasis on its role in the central regulation of energy balance. J Chem Neuroanat 2014;61–62:248–55.10.1016/j.jchemneu.2014.08.006Search in Google Scholar PubMed
47. Kołodziej U, Maciejczyk M, Miąsko A, Matczuk J, Knaś M, Żukowski P, et al. Oxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats. Front Physiol 2017;8:20.10.3389/fphys.2017.00020Search in Google Scholar PubMed PubMed Central
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